Regulation of cyclooxygenase-2 expression in cultured mouse macula densa cells
スポンサーリンク
概要
- 論文の詳細を見る
Objectives : Kidney macula densa (MD) cells express cyclooxygenase-2 (COX-2). COX-2-derived prostaglandins (PGs) stimulate the renin release from the granular cells of afferent arterioles, especially during volume depletion. In the present study, a novel established mouse macula densa (NE-MD) cells were used to directly determine whether or not COX-2 gene expression is regulated by low luminal NaCl concentration ([NaCl]) and other cellular signals, such as cAMP and Ca^<2+>. Methods : COX-2 gene expression was evaluated by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Results : Real-time RT-PCR reaction showed a significant increase in COX-2 mRNA expression in the samples with low Cl^- solution and culture media plus furosemide (12μM), an inhibitor of Na^+-K^+-2Cl^- symporter, compared with controls (P<0.05). However, it was not observed in the samples with a low Na^+ solution. Low Cl^--induced increase in COX-2 mRNA expression was completely reversed by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA), an inhibitor of Na^+/H^+ exchanger, or BAPTA-AM, a membrane-permeable Ca^<2+> chelator. Finally, COX-2 gene expression was slightly increased in the presence of forskolin, ionomycin (Ca^<2+> ionophore) or prostaglandin E_2 (PGE_2). Conclusion : These results suggest that COX-2 gene expression may be regulated by either extracellular [NaCl] or changes in the levels of intracellular cAMP, pH, and Ca^<2+>. COX-2 may influence the tubuloglomerular feedback by generating PGE_2.
- 北里大学の論文
- 2007-03-31
著者
-
Kawahara Katsumasa
Department Of Cellular And Molecular Physiology Kitasato University Graduate School Of Medical Scien
-
Akiba Lisa
Department Of Cellular And Molecular Physiology Graduate School Of Medical Sciences Kitasato Univers
-
Yasuoka Yukiko
Department Of Cellular And Molecular Physiology Kitasato University Graduate School Of Medical Scien
-
Yasuoka Yukiko
Department Of Physiology Kitasato University School Of Medicine
-
Kawahara Katsumasa
Department Of Cellular And Molecular Physiology Kitasato University Graduate School Of Medical Scien
-
FUKUDA Hidekazu
Department of Physiology, Kitasato University School of Medicine
-
Yasuoka Yukiko
Department Of Cellular And Molecular Physiology Kitasato University Graduate School Of Medical Scien
-
Fukuda Hidekazu
Department Of Physiology Kitasato University School Of Medicine
-
Fukuda Hidekazu
Department Of Biological Sciences Tokyo Institute Of Technology
関連論文
- Tumor Necrosis Factor-α Downregulates the Voltage Gated Outward K^+ Current in Cultured Neonatal Rat Cardiomyocytes : A Possible Cause of Electrical Remodeling in Diseased Hearts(EXPERIMENTAL INVESTIGATION)
- Establishment of a Mouse Macula Densa Cell Line with an nNOS Promoter Driving EGFP Expression
- Folliculogenesis following syngeneic transplantation of young murine ovaries into the testes
- Localization of the Sulphonylurea Receptor Subunits, SUR2A and SUR2B, in Rat Renal Tubular Epithelium
- Development of Renal Potassium Excretion Capacity in the Neonatal Rat
- Effect of Ion Transport Inhibitors and Methacholine on Short-Circiut Current of Isolated Guinea Pig Nasal Epithelium
- Cre-loxP System Confers Cell Lineage-Specific Expression of a Reporter Gene in Murine Preimplantation Development
- Potassium Transport and Potassium Channels in the Kidney Tubules
- Upregulation of calbindin D28k in the late distal tubules in the potassium-loaded adrenalectomized mouse kidney
- Hypotonicity-Induced ATP Release Is Potentiated by Intracellular Ca^ and Cyclic AMP in Cultured Human Bronchial Cells
- Roles of Vasopressin and Hypertonicity in Basolateral Na/K/2Cl Cotransporter Expression in Rat Kidney Inner Medullary Collecting Duct Cells
- Low [NaCl]-induced neuronal nitric oxide synthase (nNOS) expression and NO generation are regulated by intracellular pH in a mouse macula densa cell line (NE-MD)
- Regulation of cyclooxygenase-2 expression in cultured mouse macula densa cells
- L-Type Ca^ Channels in the Enteric Nervous System Mediate Oscillatory Cl^- Secretion in Guinea Pig Colon
- MARCH-III Is a Novel Component of Endosomes with Properties Similar to Those of MARCH-II
- Renal Compensation for Body Water Loss during Dehydration in Neonatal Rats
- The kidney and hypertension