Rapid Detection of Yeast in Orange Juice by Enzyme-linked Immunosorbent Assay(Food & Nutrition)

元データ 1991-12-23 社団法人日本農芸化学会


Antisera against native yeast of 6 species, Candida intermedia, C. parapsilosis, C. guilliermondii, C. lambica, Crytococcus laurentii, and Rhodotorula rubra, were prepared for use as probes in an enzyme-linked immunosorbent assay (ELISA). These antisera reacted with yeast cell surface antigens that are thought to consist of protein moieties. The cross-reactivities between the antisera and yeast of 11 species were investigated by immunofruorescent and immunoblotting methods. It was shown that ELISA using the antiserum against C. intermedia or C. parapsilosis, is a valuable means of detecting yeast in orange juice. Yeast of more than 10^3 were capable of being detected by ELISA.


MAEDA Hisao Wakayama AGRI-BIO Research Center
IFUKU Yasushi Wakayama AGRI-BIO Research Center
Maeda Hisao Wakayama Agricultural Biological Insititute Center
Ifuku Yasushi Wakayama Agricultural Biological Insititute Center
Yoshida Motonobu Research Institute of Food Science, Kinki University
Yoshida Motonobu Research Institute Of Food Science Kinki University