Purification and Characterization of Rice Peroxidases(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• Four peroxidase components, named RP-2, 4, 6, and 7, were isolated from rice (Oryza saliva L.) green leaves. Isoelectric focusing indicated that each preparation was homogeneous. The molecular weights of RP-2, 4, 6, and 7 estimated by SDS-PAGE were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. The activity of every preparation was maximum around pH 5.0. Antisera against these purified enzymes were raised in rabbits. Ouchterlony double diffusion tests with these antisera suggested that RP-6 and 7 were immunochemically identical and RP-2 and 4 were identical in parts and that RP-6 and 7 were quite different from RP-2 and 4. Analysis of the N-terminal amino acid sequences also showed that these peroxidase components were classified into two groups. The polymerase chain reaction showed that RP-2 and/or RP-4 contained an active central region, which is homologous to other plant peroxidases.

• 社団法人日本農芸化学会の論文
• 1991-10-23

著者

• Hiraoka Nobutsugu

  Bioproducts Development Center Takara Shuzo Co. Ltd.

• Ohashi Yuko

  Department Of Molecular Biology National Institute Of Agrobiological Resources

• ITO Hiroyuki

  Bioproducts Development Center, Takara Shuzo Co., Ltd.

• OHBAYASHI Akira

  Central Research Laboratories, Takara Shuzo Co., Ltd.

• Ohbayashi Akira

  Central Research Laboratories Takara Shuzo Co. Ltd.

• Ito Hiroyuki

  Bioproducts Development Center Takara Shuzo Co. Ltd.

関連論文

• A Large Family of Class III Plant Peroxidases
• Wound-Induced Expression of a Tobacco Peroxidase Is Not Enhanced by Ethephon and Suppressed by Methyl Jasmonate and Coronatine
• Ethylene Promotes the Necrotic Lesion Formation and Basic PR Gene Expression in TMV-Infected Tobacco
• Involvement of Proteasome-Ubiquitin System in Wound-Signaling in Tobacco Plants
• Accumulation of Pathogenesis-Related Proteins in Tobacco Leaves Irradiated with UV-B
• イネペルオキシダ-ゼ遺伝子のRFLPマッピング--アイソザイム分析によるものとは異なる新しい1遺伝子座の検出〔英文〕
• Purification and Characterization of Rice Peroxidases(Biological Chemistry)
• Induction of Salicylic Acid β-Glycosides in Tobacco Leaves by Exogenous Salicylic Acid : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
• Stress-Induced Expression of Genes for Pathogenesis-Related Proteins in Plants : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION
• Generation of Dimethyl Sulfide from Dimethyl-β-propiothetin in an Extract of Green Alga Monostroma nitidum and Its Retention by Cyclodextrin(Food & Nutrition)

スポンサーリンク