Cloning and Identification of a cDNA from Rat Liver Coding for a Portion of L-Gulonolactone Oxidase(Biological Chemistry)

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概要

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• An enzyme, L-gulono-γ-lactone oxidase, was purified from the livers of male albino rats and used to prepare a polyclonan rabbit antiserum. Reaction of the enzyme in cell-free extracts with the antiserum gave a single precipitin line when tested by the double diffusion method on agarose plates. Using the purified enzyme, the antiserum could detect nanogram quantities of the enzyme in dot-blotting procedures. This antiserum was used to screen a rat liver cDNA library in the vector λgtll. Positively reacting clones could easily be identified. One clone contained a cDNA insert of 800 bp and produced a β-galactosidase fusion protein with a MW 30,000 greater than the native molecule.

• 社団法人日本農芸化学会の論文
• 1989-04-23

著者

• Hong Bum-shik

  Institute Of Biotechnology Korea University

• Seib Paul

  Department Of Grain Science And Industry

• YANG H.-C.

  Institute of Biotechnology, Korea University

• MARCHIN George

  Bioserve SPace Technologies (a NASA CDS Center), Section on Microbiology, Division of Biology, Kansa

• Yang H.-c.

  Institute Of Biotechnology Korea University

• Marchin George

  Bioserve Space Technologies (a Nasa Cds Center) Section On Microbiology Division Of Biology Kansas S

関連論文

• Lipase-catalyzed Synthesis of C-6 Saturated and Unsaturated Fatty Acid Esters of L-Ascorbic Acid
• Cloning and Identification of a cDNA from Rat Liver Coding for a Portion of L-Gulonolactone Oxidase(Biological Chemistry)

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