Production of Amylase and Pullulanase by an Alkalopsychrotrophic Micrococcus sp.(Microbiology & Fermentation Industry)

概要

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An alkalopsychrotrophic strain, Micrococcus sp. 207, inducibly and extracellulary produced amylase and pullulanase. The main hydrolysis product from amylose, with a crude enzyme preparation, was maltotetraose. The optimum temperature for activity of the amylase was 60℃ and that for pullulanase 55℃. The activities at 0 to 30℃ exhibited similar activation energy values. In an optimized production medium at pH 9.7, the highest yields of these enzymes were obtained after cell growth at 18℃ for 4 days. At pH 8.5, the yields of amylase and pullulanase became maximum after 3 days cultivation. With more prolonged cultivation, the yield of amylase but not that of pullulanase activity decreased. These enzymes were not produced at temperatures above 30℃. Sucrose was not effective as an inducer, but it stimulated cell growth and enhanced the enzyme productivities with soluble starch.
社団法人日本農芸化学会の論文
1989-11-23