Isolation and Partial Characterization of a New Manganoprotein in Soybeans (Food & Nutrition)

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概要

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• The isolation and partial characterization of a tightly-bound-manganese containing protein in soybean seeds were performed. Sephadex G-100 rechromatography of the manganese fraction from the aqueous supernatant of soybeans showed one manganese peak corresponding to a molocular weight of 61,000. Upon DEAE-Sephadex A-25 chromatography at pH 7.4, the manganese fraction was separated into two components; component I did not bint to the resin while component II did. Components I and II both comtained zinc. The ratio of manganese to zinc contained was about 2 to 1 in both components. Components I and II showed identical absorption spectra with maximum absorpition at 278 nm. The molecular weights of the two components were estimated to be the same, 63,000. on SDS-polyacrylamide gel electrophoresis. Component I was found to be a dimer composed of two heterogeneous subunits. with molecular weighets of 32,000 and 30,500, on SDS-polyacrylamide gel electrophoresis. Component II was also revealed to be a dimer composed of two heterogeneous subunits, with molecular weights of 32,000 and 31,000.

• 社団法人日本農芸化学会の論文
• 1988-09-23

著者

• Yoshida Seisaku

  Osaka Prefectural Institute of Public Health

関連論文

• Residual Status of Chlorpyrifos and Octachlorodipropylether in Ambient Air and Polished Rice Stock in Houses Five Years after Application for Termite Control
• Distribution of the Molecular Weight and Chemical Form of Intrinsic Iron Compounds in Soybeans(Food & Nutrition)
• Distribution of Molecular Weight and Chemical Form of Manganese Compounds in Soybeans (Food & Nutrition)
• Isolation and Partial Characterization of a New Manganoprotein in Soybeans (Food & Nutrition)

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