Phenylalanine Ammonia-lyase of Bamboo Shoots (Biological Chemistry)

概要

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Phenylalanine ammonia-luase (PAL) was purified from bamboo shoots (Bambusa oldhami Munro) through steps of DEAE-Sepacel column chromatography, Shparose CL-6B gel filtration, and a second DEAE-Shepacel column chromatography. Polyacrylamide gel electrophoresis showed that the purified enzyme (M_r 86,000) still contained two minor bands with much slower mobilities (M_r ⩾660,000) than the major one. The minor band could be the aggregated forms of native PAL, based on the fact that only a single band (M_r 86,000) was observed upn sodium dodecyl sulfate-polyacrylamide gel electro phoresis or isoelectric focusing, and all bands shared common antigenic determinants as revealed by immunoblotting done with an antiserum raised against the major band. There was a sharp rise of PAL activity of bomboo shoots after harvest, and this was only partly due to the de novo synthesis of this enzyme as measured by Laurell's rocket immunoeletrophoresis.
社団法人日本農芸化学会の論文
1988-09-23