Efficient Usage of a Galactose-inducible Expression Vector for the Production of Heterologous Protein in Yeast(Microbiology & Fermentation Industry)

スポンサーリンク

概要

• 論文の詳細を見る

• We found that the expression vector previously constructed using the promoter of the GAL7 gene of Saccharomyces cerevisiae lacked the transcription terminator. The addition of the GAL7 terminator resulted in a more than 3-fold increase in the production of the protein encoded by cDNA of early region 1a (E1a) of human adenovirus, without affecting the amount of its mRNA. The positive regulatory gene, GAL4, when amplified with a vector of a high copy number, caused an about 5-fold increase in E1a production. We also found that medium containing a mixture of galactose and glucose can be used for the efficient production of the E1a protein.

• 社団法人日本農芸化学会の論文
• 1988-08-23

著者

• Wada T

  Yamaguchi Univ. Yamaguchi Jpn

• ABE Akio

  Laboratory of Molecular Genetics, Keio University School of Medicine

• WADA Tadashi

  Laboratory of Molecular Genetics, Keio University School of Medicine

• HANDA Hiroshi

  Laboratory of Molecular Genetics, Keio University School of Medicine

• NOGI Yasuhisa

  Laboratory of Molecular Genetics, Keio University School of Medicine

• FUKASAWA Toshio

  Laboratory of Molecular Genetics, Keio University School of Medicine

• Abe Akio

  Laboratory Of Bacterial Infection Kitasato Institute For Life Sciences Kitasato University

• Fukasawa Toshio

  Laboratory Of Molecular Genetics Keio University School Of Medicine

• Abe A

  Univ. British Columbia Vancouver Can

• Handa Hiroshi

  Laboratory Of Molecular Genetics Keio University School Of Medicine:(present Office)department Of Mi

• Nogi Yasuhisa

  Laboratory Of Molecular Genetics Keio University School Of Medicine

関連論文

• Efficient Usage of a Galactose-inducible Expression Vector for the Production of Heterologous Protein in Yeast(Microbiology & Fermentation Industry)
• The Bordetella type III secretion system : its application to vaccine development
• EspJ effector in enterohemorrhagic E. coli translocates into host mitochondria via an atypical mitochondrial targeting signal

スポンサーリンク