Increased Expression in Escherichia coli of Human Tumor Necrosis Factor through in Vitro Mutagenesis around the Initation Codon(Biological Chemistry)

概要

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This study was done to investigate the effects of modified nucleotide sequences around the initiation codon on gene expression efficiency in Escherichia coli. A human tumor necrosis factor-β-galactosidase fused gene was used to randomly modify both the upstream nucleotide sequence from the initiation codon (-4 to -1) and the silent third letter of the codons corresponding to the 4 amino acid residues of the N-terminal region. It was observed that the frequency of adenine nucleotide usage in this region was higher in the high expression group than in the low expression group.
社団法人日本農芸化学会の論文
1988-06-23