Characterization of Cell-wall-bound Nuclease and Ribonuclease from Potato Tuber(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• Two enzymes, a nuclease and a ribonuclease (RNase) were isolated from the cell walls of potato tubers. The nuclease was purified by 1370-fold fractionation with ammonium sulfate and chromatography on Sephadex G-200 and DEAE-Sephadex A-50 columns. The purified nuclease hydrolysed both RNA and DNA, yielding primarily purine 5'-nucleotides. The ribonuclease did not cleave DNA and the hydrolysis of RNA produced 2':3'-cyclic nucleotides as products. Both enzymes were inhibited by Cu^<2+> and Zn^<2+>, while EDTA inhibited the nuclease and promoted the RNase. The cell wall enzymes are different from the corresponding cytoplasmic enzymes previously characterized with respect to pH and temperature optima and molecular weights.

• 社団法人日本農芸化学会の論文
• 1988-04-23

著者

• Palcic M

  Univ. Alberta Alberta Can

• Palcic M.

  Department Of Food Science University Of Alberta:(present Office)department Of Applied Biological Sc

• NGUYEN T.

  Department of Food Science, University of Alberta

• HADZIYEV D.

  Department of Food Science, University of Alberta

• Hadziyev D.

  Department Of Food Science University Of Alberta

• Nguyen T.

  Department Of Food Science University Of Alberta

関連論文

• Rapid Enzymatic-HPLC Measurement of the Content of Major Nucleotides in Vegetables(Food & Nutrition)
• Characterization of Cell-wall-bound Nuclease and Ribonuclease from Potato Tuber(Biological Chemistry)
• AN AXISYMMETRIC MODEL FOR ULTIMATE CAPACITY OF A SINGLE PILE IN SAND

スポンサーリンク