Generation of Monoclonal Antibodies Specific for Human Hemoglobin(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• One monoclonal antibody (MoAb) highly specific for human hemoglobin, KM-371, was generated by a unique immunization procedure using mica rendered tolerant to bovine hemoglobin. KM-371 strongly reacted with human hemoglobin, but did not with bovine, horse, goat, sheep, rabbit, porcine, chicken, and fish hemoglobins. Human hemoglobin was able to be quantitatively measured in the range 0.01〜200μg/ml by a sandwich ELISA using KM-371 and biotinylated KM-371. By immunostaining using KM-371, 20ng of human hemoglobin could be detected on a nitrocellulose membrane. These results suggest that KM-371 would be a useful MoAb in the test of occult bloods in faeces for diagnosing colorectal carcinoma without a meat diet.

• 社団法人日本農芸化学会の論文
• 1988-11-23

著者

• Kusano Akira

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.

• YOSHIDA Hajime

  Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.

• Yoshida H

  Kyowa Hakko Kogyo Co. Ltd. Tokyo Jpn

関連論文

• Generation of Monoclonal Antibodies Specific for Human Hemoglobin(Biological Chemistry)
• Production of Lactase by Mutants of Kluyveromyces lactis(Microbiology & Fermentation Industry)
• L-Arginine Production by Arginine Analog-resistant Mutants of Microorganisms

スポンサーリンク