Simple Measurement of Gizzerosine in Fish Meals by High-performance Liquid Chromatography(Analytical Chemistry)

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概要

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• A simple method has been developed for analysis of gizzerosine in fish meals. A sample, hydrolyzed with 6N HCI, was transferred into an Amberlite IR CG-50 column which was equilibrated wiht sodium borate solution (0.045M, pH8.25). After elimination of a large portion of amino acids from the column, gizzerosine was eluted with 0.5N NH_4OH and then measured by HPLC. HPLC separation was done with an analytical column of Hitachi 2619-F and gradient elution with three solvents (two borate buffers-pH 9.2 and 10.7,[Na^+]=0.1M, containing 3% (v/v) EtHO-and 0.8% NaOH). Gizzerosine was measured by the post-column reaction of OPA and fluorometric detection. Recovery of gizzerosine added to fish meal was over 90% and the detection limit was 1 ppm.

• 社団法人日本農芸化学会の論文
• 1988-11-23

著者

• Ohta Y

  Tochigi Research Laboratories Of Kao Corporation

• Machida Yoshiaki

  Central Research Laboratory Showa Sangyo Co. Ltd.

• OHTA Yoshio

  Central Research Laboratory, Showa Sangyo Co., Ltd.

• OHASHI Hideo

  Central Research Laboratory, Showa Sangyo Co., Ltd.

• ENOMOTO Setsuko

  Central Research Laboratory, Showa Sangyo Co., Ltd.

• Enomoto Setsuko

  Central Research Laboratory Showa Sangyo Co. Ltd.

• Ohashi Hideo

  Central Research Laboratory Showa Sangyo Co. Ltd.

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• Enhanced Production of Extracellular Enzymes by Mutants of Bacillus That Have Acquired Resistance to Vancomycin and Ristocetin(Microbiology & Fermentation Industry)
• Simple Measurement of Gizzerosine in Fish Meals by High-performance Liquid Chromatography(Analytical Chemistry)

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