An Attempt at Measuring Saccharose Flux Permeating through the Oil Layer in W/O/W Emulsions on the Basis of Hydrolytic Activity of Invertase(Food & Nutrition)

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A study was done to measure the saccharose flux permeating through the oil layer of the dispersed vesicular globules in a series of W/O/W-type multiple emulsions, considering in connection with the effect of entrapped invertase on the hydrolytic activity in the vesicular globules. The W/O/W emulsions containing a definite quantity of invertase in the inner aqueous phase of the globules were prepared using a mixture of Span 80 and SDS, and were dialyzed against an aqueous solution of 0.1 M saccharose for over 10 days at 25℃. Thus, it was possible to follow an increasing pattern of reducing sugar with each sample in the dialysis container according to the hydrolysis of saccharose, which was diffusing into the inner aqueous phase from the outer aqueous medium permeating through the oil layer. A range of values from 1.5 to 11.2 PM sec^<-1> were observed for the molar flux of saccharose against 1 cm^2 of the oil layer. Although the values obtained were scattered within the above range due to the variations of the samoles, the hydrolytic activity of invertase seemed to be kept in the vesicular globules during a span of the dialyzing pocedure.
社団法人日本農芸化学会の論文
1988-11-23