Purification of an Aromatic Amine-dependent NAD(P)H Oxidase from Tomato Fruit and Its Characterization as a Peroxidase(Biological Chemistry)
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概要
- 論文の詳細を見る
1. An aromatic amine-dependent NAD(P)H-oxidizing enzyme was purified from tomato fruit to homogeneity as judged by SDS-polyacrylamide gel electrophoresis. NAD(P)H was oxidized under aerobic conditions in the presence of aromatic amines such as N,N-dimethylaniline, N-methylaniline, and aniline. The amines were essential for the reaction, but were not metabolized. 2. The enzyme had a monomer molecular weight of 48,000. On the basis of its spectral characteristics and the inhibition by KCN, NaN_3, and catalase, the enzyme was identified as a protoheme peroxidase.
- 社団法人日本農芸化学会の論文
- 1988-10-23
著者
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Omura Tsuneo
Department Of Biochemistry Vanderbilt University School Of Medicine
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SHIGEMATSU HIDENARI
Daiichi Colledge of Pharmaceutical Sciences
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Shigematsu H
Daiichi College Of Pharmaceutical Sciences
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Omura Tsuneo
Department Of Molecular Biology Graduate School Of Medical Science Kyushu University
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KUWAHARA Toyoko
Daiichi College of Pharmaceutical Sciences
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Kuwahara T
Daiichi Coll. Pharmaceutical Sciences Fukuoka Jpn
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