Construction of an Expression and Secretion Vector for the Yeast Saccharomyces cerevisiae(Biological Chemistry)

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概要

• 論文の詳細を見る

• In order to make yeast cells secrete useful materials, an expression and secretion vector was constructed using the promoter and the signal sequence of yeast invertase coded by the SUC2 gene. When mouse amylase, after removal of its own signal sequence, was ligated with the secretion vector pSMF38T, amylase activity was secreted by the yeast Saccharomyces cerevisiae. The amount of the secreted enzyme was estimated to be 600 μg/liters culture under the derepression conditions for the SUC2 promoter function.

• 社団法人日本農芸化学会の論文
• 1987-02-23

著者

• Nishizawa Masafumi

  Laboratory Of Molecular Genetics Mitsubishi-kasei Institute Of Life Sciences:(present Office)bioscie

• Hishinuma Fumio

  Laboratory Of Molecular Genetics Mitsubishi-kasei Institute Of Life Sciences

• Hishinuma Fumio

  Laboratories Of Molecular Genetics Mitsubishi Kasei Institute Of Life Sciences

• OZAWA Fumiko

  Laboratory of Molecular Genetics, Mitsubishi-Kasei Institute of Life Sciences

• Ozawa Fumiko

  Laboratory Of Molecular Genetics Mitsubishi-kasei Institute Of Life Sciences

関連論文

• Characterization of Mouse α-Amylase Secreted from Saccharomyces cerevisiae by the pGKL 128kDa Killer Secretion Signal
• Construction of an Expression and Secretion Vector for the Yeast Saccharomyces cerevisiae(Biological Chemistry)

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