Improvement of Histidine-producing Strains of Serratia marcescens by Cloning of a Mutant Allele of the Histidine Operon on a Mini-F Plasmid Vector(Microbiology & Fermentation Industry)

概要

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Two hybrid plasmids bearing a mutant allele of the histidine operon of Serratia marcescens on a mini-F plasmid vector were constructed: one was plasmid pSS503 harboring the 4.7-kb ecoRI fragment bearing the initial part of the histidine operon and the other was plasmid pSH368 harboring the 12-kb EcoRI-BamHI fragment bearing the whole histidine operon. Both plasmids increased the activities of ATP-phosphoribosyltransferase (hisG product), histidinol dehydrogenase (hisD product) and histidinol phosphate phosphatase (hisB product) about two-fold, as compared with those observed with the plasmid-free host strain, a histidine-producing strain, L120, of Serratia marcescens. both plasmids were considerably unstable in the histidine-producing strain, although they were completely stable in the wild-type strain. Strain L120 carrying either pSS503 or pSH 368 produced 42mg/ml or L-histidine; this productivity was approximately 50% more than that of plasmid-free strain L120.
社団法人日本農芸化学会の論文
1987-02-23