Effect of Phytic Acid on the Hydrolysis of Lactose with β-Galactosidase(Food & Nutrition)

概要

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The effects of phytate on the hydrolysis of lactose with β-galactosidases from bovine liver and Escherichia coli were investigated. The activities of both β-galactosidases were decreased to the same extent by increased concentrations of phytate. The rates of inhibition of β-galactosidases activity from E. coli in a reaction mixture containing 10 mM of phytate were 78.9% and 64.4%, respectively, in the absence of and with 4 mM of Mg^<2+>. Therefore, it was found that the stimulatory effect of Mg^<2+> was hardly affected by the presence of phytate in the range from 2 to 10 mM. The β-galactosidases activity was also not influenced by preincubating β-galactosidase or lactose with phytate. Kinetic studies showed that the inhibition of β-galactosidase activity by phytate was of an uncompetitive type with a Ki value of 3.46mM. Therefore, it is considered that phytate may interact with a complex of β-galactosidase and lactose.
社団法人日本農芸化学会の論文
1987-11-23