Expression of Chemically Synthesized α-Neo-endorphin Genes under the Control of the PH05 Gene of Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
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概要
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A chemically synthesized α-neo-endorphin (αNE) gene was fused to the PH05 gene which encodes repressible acid phosphatase (APase) of Saccharomyces cerevisiae. Three kinds of genes encoding APase-αNE chimeric proteins were cloned in the yeast. Nucleotide sequence analysis revealed that C-terminal amino acids comprising 6 to 17% of the intact enzyme were replaced by αNE. The APase-αNE chimeric proteins were expressed at the level of approximately 1×10^6 molecules per cell under the derepressed conditions. The radioimmunoassay for αNE revealed that most of the APase-αNE chimeric proteins was not present in the periplasmic fraction but in the protoplast fraction.
- 社団法人日本農芸化学会の論文
- 1986-05-23
著者
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Nakazato Hiroshi
Suntory Institute For Biomedical Research
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TANAKA SHOJI
Suntory Institute for Biomedical Research
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OSHIMA Takehiro
Suntory Institute for Biomedical Research
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ARIMA Kenji
Suntory Institute for Biomedical Research
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MATSUBARA Kumiko
Suntory Institute for Biomedical Research
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- Expression of Chemically Synthesized α-Neo-endorphin Genes under the Control of the PH05 Gene of Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
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