Construction of a p-Nitrophenolate-Sensitive Membrane Electrode and Its Application to an Enzyme Assay(Analytical,Chemical)
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概要
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A p-nitrophenolate-sensitive membrane electrode was constructed by using tetraheptyl-ammonium chloride as an ion-exchanger and о-nitrophenyl phenyl ether as a membrane solvent. The electrode responded to p-nitrophenolate down to 1 μM in a buffer solution of 0.1 M Na_2CO_3-NaHCO_3 (pH 10). The selectivity coefficients of the electrode to typical anions increased in the following order: Cl^- <NO_^-3<SCN^-≈p-nitrophenoIate<ClO_4^-. The method was applied to determine the enzyme activity of alkaline phosphatase.
- 公益社団法人日本薬学会の論文
- 1987-09-25
著者
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Katsu Takashi
Faculty Of Pharmaceutical Sciences Okayama University
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KANAMITSU MIEKO
Faculty of Pharmaceutical Sciences, Okayama University
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FUJITA YAZABURO
Faculty of Pharmaceutical Sciences, Okayama University
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Kanamitsu M
Faculty Of Pharmaceutical Sciences Okayama University
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Katsu Takashi
Faculty Of Medicine Dentistry And Pharmaceutical Sciences Okayama University
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KATSU TAKASHI
Faculty of Pharmaceutical Sciences, Okayama University
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