Transgenic Tomato Plants that Overexpress a Gene for NADH-dependent Glutamate Dehydrogenase (legdh1)

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概要

• 論文の詳細を見る

• Tomato plants were transformed with a plasmid that included a legdh1 gene for NADH-dependent glutamate dehydrogenase (NADH-GDH) from Lycopersicon esculentum Mill, coupled, in the sense orientation, with the constitutively active 35S promoter from cauliflower mosaic virus. Three independent transformants were obtained. In these transgenic lines, high-level expression of legdh1 mRNA was detected in the leaves, and the NADH-GDH activity in the leaves of the transgenic plants was approximately twice that in the leaves of the non-transgenic plant. In the transgenic tomato fruits examined for six successive weeks after flowering, the levels of total free amino acids were higher (2.1-to 2.3-fold) than those in the controls. In particular, the level of glutamate was about twice that in the control fruits.

• 日本育種学会の論文

著者

• Miwa Tetsuya

  Research Institute For Health Fundamentals Ajinomoto Co. Inc.

• Kisaka Hiroaki

  Research Institute For Health Fundamentals Ajinomoto Co. Inc.

• Kida Takao

  Basic Research Laboratory Ajinomoto Co. Inc.:(present Office)life Science Laboratory Ajinomoto Co. I

• Kida Takao

  Basic Research Laboratories Central Research Laboratories Ajinomoto Co. Inc.

関連論文

• 酵素液中に添加したチオ硫酸銀(STS)によるシンテッポウユリのプロトプラストの分裂率の向上
• Transgenic Tomato Plants that Overexpress a Gene for NADH-dependent Glutamate Dehydrogenase (legdh1)
• Antisense suppression of glutamate decarboxylase in tomato (Lycopersicon esculentum L.) results in accumulation of glutamate in transgenic tomato fruits

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