Antisense suppression of glutamate decarboxylase in tomato (Lycopersicon esculentum L.) results in accumulation of glutamate in transgenic tomato fruits

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概要

• 論文の詳細を見る

• Tomato plants were transformed with a plasmid that contained a the gene for glutamate decarboxylase (GAD) from Lycopersicon esculentum L. coupled, in the antisense orientation, with the constitutively active 35S promoter from cauliflower mosaic virus. Four independent transformants were obtained. In the fruits of these transgenic plants, the level of expression of GAD mRNA was lower than that in non-transgenic plants. When tomatoes were harvested six weeks after the first flowering, we found that the levels of total free amino acids in transgenic fruits were 1.2 to 3.2 times higher than those in non-transgenic plants. In particular, the level of glutamate in fruits of transgenic plants was about twice that in fruits of non-transgenic plants.

• 日本植物細胞分子生物学会の論文
• 2006-06-01

著者

• Miwa Tetsuya

  Research Institute For Health Fundamentals Ajinomoto Co. Inc.

• Kisaka Hiroaki

  Research Institute For Health Fundamentals Ajinomoto Co. Inc.

• Kida Takao

  Basic Research Laboratory Ajinomoto Co. Inc.:(present Office)life Science Laboratory Ajinomoto Co. I

• Kida Takao

  Basic Research Laboratories Central Research Laboratories Ajinomoto Co. Inc.

関連論文

• 酵素液中に添加したチオ硫酸銀(STS)によるシンテッポウユリのプロトプラストの分裂率の向上
• Transgenic Tomato Plants that Overexpress a Gene for NADH-dependent Glutamate Dehydrogenase (legdh1)
• Antisense suppression of glutamate decarboxylase in tomato (Lycopersicon esculentum L.) results in accumulation of glutamate in transgenic tomato fruits

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