フローサイトメトリーを用いたシクラメン植物体および花粉の倍数性検定

概要

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Rapid determination of ploidy levels in cyclamen by using flow cytometry of DAPI-stained nuclei was examined. A strong positive linear correlation between DAPI fluorescent intensities of nuclei extracted from the leaf blades of cyclamen plants and the ploidy levels in diploid, triploid and tetraploid plants was confirmed. Thus, the flow cyto-metry of DAPI-stained nuclei in cyclamen was proven to be an effective and easy tool to determine the ploidy levels within a short time. DAPI fluorescent intensities of nuclei extracted from the pollen grains of diploid plants having and without giant pollen grains were also investigated. Three peaks of 1C, 2C and 4C were observed in the plants having giant pollen grains, whereas only 1C and 2C peaks were observed in the plants without giant pollen. The results suggested that the giant pollen grains were unreduced 2n pollen grains.
香川大学の論文
2007-02-28