20301 マイクロチャンネル表面の機能発現と生化学分析への応用(OS14 機能性マイクロデバイスの創製・評価)
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Proteins were immobilized on the inner wall of Polymeric micro-channels using both photo-chemical reaction and physical adsorption. Photochemical procedure enabled the accumulation of protein functions in a very small space of the chip by the immobilization of several enzymes as well as antibodies. In this lecture, some example of the device will be demonstrated. 1. Bovine serum albumin was first adsorbed on the PDMS micro channel, then Glucose oxydase (GOD) and HRP modified with photo-chemical cross linker (ATFB-SE). which had both photo-reactive moiety at one end and amine reactive group at the other was immobilized on BSA at the different position of microchannel. The chip detected glucose by the following scheme: glucose+oxygen+GOD gave glucronic acid+hydrogen peroxide, then hydrogen peroxide+HRP+Amplex red gave Resorufin (highly fluorescent). The similar procedure gave immune reactive sensor chip.
- 一般社団法人日本機械学会の論文
- 2005-03-17
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