結紮および菌破壊液の滴下により発生したサルの実験的歯周炎

概要

論文の詳細を見る
The purpose of the present investigation was to study the clinical and histopathological aspects in the progression of a periodontal lesion starting from a healthy periodontium. One monkey weighing 3 kilos was used. During a pre-experimental period of 3 weeks the teeth of the monkey were cleaned once daily with toothbrush and rubber tip. At the end of the preparatory period the gingivae were in excellent clinical health and they were at quadrant divided into experimental and control groups. The silk ligatures were placed around the premolars and the molars on each side of the maxilla and a topical application of the sonicates from Actinomyces viscosus and Fusobacterium nucleatum was used in the mandibular left and the maxillary right sides for 4 hours once a week. The mandibular right side served as a control which was subjected to meticulous toothbrushing. The plaque accumulation, the gingival inflammation, the pocket depth and the distance from the reference point to the gingival crest were assessed at regular intervals during an 3 month experimental period. The animal was sacrificed after 3 months and a serial section of the premolars and the molars with surrounding tissues was prepared for the light microscopic examination. In the sections were measured (1) the size of the infiltrated connective tissue (ICT), (2) the distance from the cemento-enamel junction (CEJ) to the apical cells of the junctional epithelium (JE), and (3) the distance from CEJ to the marginal bone crest (BC). The following findings were obtained: 1) At the start of the study no plaque and gingival inflammation could be detected. The gingivae around the teeth showed the presence of abundant amounts of plaque and the signs of pronounced inflammation after 1 month in the ligature and the ligature plus topical application groups. The plaque formation and the gingival inflammation developed with time. The control group did not show a significant increase in the Plaque and the Gingival Indices. 2) The pocket depth in both ligature and ligature plus topical application groups rapidly more increased than that in the other groups. 3) The distance from the reference point to the gingival crest in the three groups was significantly larger than that in the control group. 4) The size of ICT occupied 3.3% in the control group, 12.3% in the topical application group, 27.7% in the ligature group and 26.7% in the ligature plus topical application group. 5) The distance from CEJ to JE was similar in the topical application and the control groups. The "histological gingival recession" in the ligature and the ligature plus topical application groups was significantly larger than that in the control group. 6) In the biopsies from the ligature and the ligature plus topical application groups it was noted that BC was located slightly below the level in the control group. It is apparent from the present study that the silk ligature and the topical application of the sonicates from oral microorganisms play an important role in the initiation of periodontitis.
特定非営利活動法人日本歯周病学会の論文
1980-06-28

結紮および菌破壊液の滴下により発生したサルの実験的歯周炎

スポンサーリンク

概要

著者

関連論文

スポンサーリンク