Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head(Technical Advancement)
スポンサーリンク
概要
- 論文の詳細を見る
Hard tissue decalcification can cause variation in the constituent protein characteristics. This paper describes a method of preparating of frozen mouse head sections so as to clearly observe the nature of the constituent proteins. Frozen sections of various green fluorescent protein (GFP) transgenic mouse heads were prepared using the film method developed by Kawamoto and Shimizu. This method made specimen dissection without decalcification possible, wherein GFP was clearly observed in an undamaged state. Conversely, using the same method with decalcification made GFP observation in the transgenic mouse head difficult. This new method is suitable for observing GFP marked cells, enabling us to follow the transplanted GFP marked cells within frozen head sections.
- 日本組織細胞化学会の論文
著者
-
Aizawa Tomoyasu
Division of Biological Science, Graduate School of Science, Hokkaido University
-
Demura Makoto
Division of Life Science, Graduate School of Life Science, Hokkaido University
-
Demura Makoto
Division Of Biological Sciences Graduate School Of Science Hokkaido University
-
Kawano Keiichi
東京大学 物理学専攻
-
MIZUGUCHI Mineyuki
Faculty of Pharmaceutical Sciences, Toyama University
-
Kawano Keiichi
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
-
Mori Yoshihiro
Faculty Of Pharmaceutical Sciences Toyama Medical Pharmaceutical University
-
Mori Yoshihiro
Faculty Of Pharmaceutical Sciences University Of Toyama
-
Demura Makoto
Hokkaido University
-
Tada Masahito
Faculty Of Pharmaceutical Sciences Univ Of Toyama
-
SHINODA Hiroyuki
Faculty of Pharmaceutical Sciences, University of Toyama
-
Shinohara Yoshinori
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
-
Kato Ichiro
The Department of Biochemistry, Toyama Medical and Pharmaceutical University
-
Hiraga Koichi
The Department of Biochemistry, Toyama Medical and Pharmaceutical University
-
Hiraga Koichi
The Department Of Biochemistry Toyama Medical And Pharmaceutical University
-
Shinohara Yoshinori
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University:tooth Regeneration D
-
Shinoda Hiroyuki
Faculty Of Pharmaceutical Sciences University Of Toyama
-
Shinoda Hiroyuki
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
-
Kato Ichiro
The Department Of Biochemistry Toyama Medical And Pharmaceutical University
-
Mizuguchi Mineyuki
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
-
Kawano Keiichi
Faculty of Advanced Life Science, Hokkaido University
-
Shinoda Hiroyuki
Faculty of Pharmaceutical Science, Toyama Medical and Pharmaceutical University
関連論文
- 3P-013 β2ミクログロブリンの天然状態におけるプロリン異性化反応の解析(蛋白質・物性(3),第46回日本生物物理学会年会)
- 3P-020 相同蛋白質のフォールディング機構 : ヤギα-ラクトアルブミンとイヌミルクリゾチームの比較研究(蛋白質・物性(3),第46回日本生物物理学会年会)
- 1P113 Backbone NMR assignment and hydrogen exchange of goat α-lactalbumin(3. Protein folding and misfolding (1),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P105 Equilibrium and kinetics of the folding of canine milk lysozyme(3. Protein folding and misfolding (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P101 The role of Ca^ in the folding of goat α-Lactalbumin(3. Protein folding and misfolding (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Heterologous Expression of Pharaonis Halorhodopsin in Xenopus laevis Oocytes and Electrophysiological Characterization of Its Light-Driven Cl- Pump Activity
- Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer : Cl- Circulation within the Extracellular Channel
- 1P-099 哺乳類消化用リゾチームのペプシン耐性と立体構造の安定性(蛋白質・物性(1),第46回日本生物物理学会年会)
- 2P313 Structural network in the extracellular region corresponding to the visual spectral shift of halorhodopsin(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P311 The role of E234 on the extracellular site in the anion pump of pharaonis halorhodopsin(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori
- 3P-097 ハロロドプシンの光活性はホモ三量体化によって変調を受けるのか?(膜蛋白質,第46回日本生物物理学会年会)
- 3P-078 酵母Pighia pastorisを用いた新規Thioredoxin融合発現系の構築(蛋白質工学,進化工学,第46回日本生物物理学会年会)
- 3P-073 不溶性顆粒を利用したペプチドの新規発現法の開発(蛋白質工学,進化工学,第46回日本生物物理学会年会)
- 2P-264 大腸菌発現系を用いたsalinarumハロロドプシンの機能解析(光生物・視覚,光受容(2),第46回日本生物物理学会年会)
- 2P-259 光駆動型クロライドポンプハロロドプシンのアニオン取込みにおけるアルギニン側鎖の構造変化(光生物・視覚,光受容(2),第46回日本生物物理学会年会)
- 2P-051 天蚕由来ヤママリンの構造・活性相関(蛋白質・構造機能相関(2),第46回日本生物物理学会年会)
- 1P-040 天然変性DNA結合モチーフSTPRの構造解析(蛋白質・構造機能相関(1),第46回日本生物物理学会年会)
- Effect of C-terminal Elongation on the Structure and Larval Growth Inhibitory Activity of Growth Blocking Peptide (GBP)
- Structural Analysis of Tachyplesin I with Dodecylphosphocholine Micelles
- The Solution Structure of Diapause-Specific Peptide
- Selection of Peptides Having a High Affinity to Hydroxyapatite with a M13 Phage Display
- Dimeric Solution Structure of Antimicrobial Peptide Tachystatin B with an Inhibitory Cystine-Knot Motif
- A Novel Cytokine from the Larval Integument of the Armyworm, Pseudaletia separata : Isolation, Activities, and NMR Solution Structure
- 2P316 The change of trimer packing intensity of halorhodopsin caused by binding or release of Cl^-ion(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P315 Trimer-monomer conversion of pharaonis halorhodopsin by anion binding(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P314 Transient movement of helices of halorhodopsin during the photocycle.(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P312 The comprehensive analysis of the color tuning residues of pharaonis halorhodopsin by site-saturation mutagenesis(41. Proton and ion pumping,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 3P-216 ハロロドプシンポンプ機能におけるシッフ塩基近傍酸性残基の役割(生体膜/人工膜・輸送,第46回日本生物物理学会年会)
- 1P512 Fast backbone amide assignment of ^N-HSQC spectrum(25. New methods and tools (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P166 A comparison of structural properties in N-terminal domain of connexin26 associated to KID syndrome(34. Membrane protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 3P002 Conformational changes of extracellular loop of halorhodopsin by chloride binding(Protein: Structure,The 48th Annual Meeting of the Biophysical Society of Japan)
- 1P255 ハロロドプシンのCl-移動中における,シッフ塩基のpKa変化(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 2P088 無脊椎動物由来抗菌ペプチドと脂質膜の相互作用解析(蛋白質-機能(反応機構,生物活性など),第48回日本生物物理学会年会)
- 1P083 ヒト由来機能未知タンパク質ZNF821における23アミノ酸からなるタンデムリピートドメインSTPRの機能解析(蛋白質-機能(反応機構,生物活性など),第48回日本生物物理学会年会)
- 3P068 イヌミルクリゾチームのアンフォールディング経路についての理論的研究 : ヤギαラクトアルブミンとの比較(蛋白質-物性(安定性,折れたたみなど),第48回日本生物物理学会年会)
- 2P057 αラクトアルブミンのMG状態と生物機能(蛋白質-物性(安定性,折れたたみなど),第48回日本生物物理学会年会)
- 3P274 A Long-lived photoproduct, P370, formed from salinarum phoborhodopsin at room temperature(Photobiology: Vision & Photoreception,The 48th Annual Meeting of the Biophysical Society of Japan)
- 3P-209 ファラオニスハロロドプシンのCl-輸送機構におけるAsp252の役割(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 3P-208 Halobacterium salinarum由来のフォボロドプシン(センサリーロドプシンII)の光化学反応サイクルとプロトン移動(光生物-視覚・光受容,第47回日本生物物理学会年会)
- 3P-205 大腸菌発現系を用いたsalinarumハロロドプシンの機能的再構成(光生物-視覚・光受容,第47回日本生物物理学会年会)
- The Structure of Physarum polycephalum Hemagglutinin I Suggests a Minimal Carbohydrate Recognition Domain of Legume Lectin Fold
- 1P-090 ヒト由来2回膜貫通型タンパク質Ifitm5の構造研究 : NMR法を用いた立体構造解析のためのサンプル調製(膜蛋白質,第47回日本生物物理学会年会)
- 1P140 A novel expression system for high-level recombinant protein production in Escherichia coli(4. Protein engineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Structural Analysis of an Antibacterial Peptide Derived from a Nematode
- Contribution of N-terminal and Loop Regions of GBP to the Interaction with Epidermal Growth Factor Receptor
- Roles of Ser130 and Thr126 in Chloride Binding and Photocycle of pharaonis Halorhodopsin
- 1P-032 新規デフェンシンの膜構造と抗菌活性(蛋白質-構造機能相関,第47回日本生物物理学会年会)
- 1TA1-09 新規デフェンシンの膜構造と抗菌活性(蛋白質-構造機能相関,第47回日本生物物理学会年会)
- 2P283 ハロロドプシン変異体S130D,S130Eの研究(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 1P118 Structural stability and photocycle of bacteriorhodopsin in short chain phospholipid micelles(Membrane proteins,The 48th Annual Meeting of the Biophysical Society of Japan)
- 3P279 膜タンパク質ハロロドプシンの構造安定性と機能へのヘリックス間ループの影響(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 3P100 共発現による封入体化を利用した効率的な短鎖ペプチド生産技術(蛋白質-蛋白質工学/進化工学,第48回日本生物物理学会年会)
- 3P006 タキプレシンI-LPS複合体の立体構造解析(蛋白質-構造,第48回日本生物物理学会年会)
- 1P264 ハロロドプシンの三量体形成メカニズムと機能変調(光生物-視覚・光受容,第48回日本生物物理学会年会)
- 1P027 細胞増殖抑制活性を持つ新規誘導体の構造および相互作用の解析(蛋白質-構造,第48回日本生物物理学会年会)
- 3P-096 蛍光相関分光法を用いたFMBP-1の生体内分子動態解析(核酸結合蛋白質,第47回日本生物物理学会年会)
- 3P-064 昆虫由来新規ケモカインHCPの解析(蛋白質-機能(反応機構,生物活性など),第47回日本生物物理学会年会)
- 3P-026 新規がん細胞増殖抑制ペプチドの構造-活性相関(蛋白質-構造機能相関,第47回日本生物物理学会年会)
- 2P-084 ハロロドプシンNpHRの三量体構造はF150残基の対称配置によって安定化される(膜蛋白質,第47回日本生物物理学会年会)
- 1P-076 共発現によるペプチド封入体化発現法におけるパートナータンパク質の検討(蛋白質-蛋白質工学/進化工学,第47回日本生物物理学会年会)
- 1P-026 α-ラクトアルブミンのMG状態と生物機能(蛋白質-構造機能相関,第47回日本生物物理学会年会)
- 2TP2-01 ハロロドプシンNpHRの三量体構造はF150残基の対称配置によって安定化される(膜蛋白質,第47回日本生物物理学会年会)
- 1TP5-09 機能未知ヒト蛋白質の23アミノ酸繰返しドメインのDNA結合性について(核酸結合蛋白質,第47回日本生物物理学会年会)
- Solution Structure of an Insect Growth Factor Peptide GBP and its EGF Activity
- Study on Structure-Activity Relationship between the N-terminal Region of S100C Protein and its Function
- Carbohydrate Binding of Gramicidin S Analogues in Aqueous Medium
- 1P-248 プロトンポンプへの機能転換をめざしたハロロドプシンの変異導入(光生物・視覚,光受容(1),第46回日本生物物理学会年会)
- 1P420 Studies on reaction dynamics of halorhodopsin during chloride ion pumping(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- 2P023 Expression and characterization of the human STPR domain : a homologue of the novel silkworm DNA-binding domain(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P022 Influence of protein structure on the adsorption of human lysozyme onto hydroxyapatite(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P325 The effect of active peptide mobility and conformation on cell morphology(11. Morphogenesis and cell adhesion,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P002 Structure of tachyplesin I bound to dodecylphosphocholine micelles(1. Protein structure and dynamics (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Solution Structures of Peptide Units from DNA Binding Domain of Silkworm Protein FMBR-1
- Effect of Molecular Assembly on Photocycle of Reconstituted Bacteriorhodopsin : Significant Blue Shift of the Late M Photointermediate in the Liquid Crystalline Phase
- 1P003 NMR solution structure of a novel cytokine from the larval integument of the armyworm, Pseudaletia separata(1. Protein structure and dynamics (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P001 Structure-activity relationships and C-terminal elongation effects of growth-blocking peptide(1. Protein structure and dynamics (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Mutational Analysis of the Small Cytokine : Growth-Blocking Peptide (GBP) to Investigate the Interaction with Its Receptor in Insect Immune Cells
- Structure-function relationship of Yamamarin from the wild silkmoth
- Amyloid Fibril Formation of a Peptide Fragment of Transthyretin
- Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head(Technical Advancement)
- 1P074 Non-native structure of α-lactalbumin-fatty acid complex as an apoptosis induction factor(2. Protein function (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 1P436 Structural Changes in the L_2-intermediate of pharaonis Halorhodopsin Studied by FTIR Spectroscopy(17. Light driven system,Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- Conformational analysis of novel peptide derivatives with cytostatic activity
- Expression of salinarum halorhodopsin in Escherichia coli cells : Solubilization in the presence of retinal yields the natural state
- 3H1458 抗腫瘍細胞活性を示すオレイン酸-蛋白質巻き戻り中間体複合体のNMR及び生物学的研究(3H 蛋白質_物性5,日本生物物理学会第49回年会)
- 2G1448 線虫由来抗菌ペプチドABF-2の発現、精製と立体構造解析(蛋白質_構造2,第49回日本生物物理学会年会)
- 2G1436 植物由来抗菌ペプチドsnakin-1の立体構造解析へ向けた研究(蛋白質_構造2,第49回日本生物物理学会年会)
- 2D1612 抗菌ペプチドhuman defensin 5前駆体の大量発現とその解析(蛋白質_構造機能相関1,第49回日本生物物理学会年会)
- 1B1512 Decay kinetics of the M photointermediate of bacteriorhodopsin in short chain phosphatidylcholine micelles(Membrane Proteins 1,The 49th Annual Meeting of the Biophysical Society of Japan)
- 1B1536 ハロロドプシンの三量体安定化に対するSer^の役割(膜蛋白質1,第49回日本生物物理学会年会)
- 1B1524 P36 atronomonas pharaonis HalorhodopsinのCl-輸送に伴う体積変化(膜蛋白質1,第49回日本生物物理学会年会)
- 1B1448 部分フッ素化フォスファチジルコリン小胞へのバクテリオロドプシンの再構成(膜蛋白質1,第49回日本生物物理学会年会)
- 2Q1512 ハロロドプシン十重変異体の研究(光生物_視覚・光受容2,第49回日本生物物理学会年会)
- Structural Analysis of Plant Antimicrobial Peptide Snakin-1
- NMR Structure and Interaction of Antimicrobial Peptide Tachyplesin I with Lipopolysaccharide
- Construction of a Novel Expression System for Cryptdin-4 by Using Inclusion Body Formation
- Does Escherichia coli Thioredoxin Improve Expression Efficiency of Recombinant Peptide in Pichia pastoris?
- Expression, Purification and NMR Characterization of the Cyclic Reconbinant Form the First Extracellular Loop of the Transmembrane Protein Halorhodopsin
- Expression and Structural Analysis of a Recombinant Antibacterial Peptide, Cecropin P1