1235 再生軟骨モデルの組織構造形成に対する培養条件の影響(G02-5 関節と軟骨(2),G02 バイオエンジニアリング)

概要

論文の詳細を見る
The dynamic mechanical loading are known to stimulate extracellular matrix synthesis of cultured chondrocytes. In this study, we developed the culture system that simulated biaxial dynamic loading applied articular cartilage in vivo. We investigated the influence of dynamic compressive loading on mechanical properties of chondrocyte-agarose construct. After 1day in static culture, constructs were exposed to cyclic uniaxial compressive strain (15%, 1Hz, 6h/day) for 7days and 14days. Then, tangent and equilibrium moduli of the specimen were obtained by unconfined compression test. The results show that the tangent modulus of compressed samples is significantly higher than control. CLSM images suggested that regeneration of collagen typeII tissue structure was stimulated under dynamic loading.
一般社団法人日本機械学会の論文
2005-09-18