Molecular Authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii by ITS and 5S rRNA Spacer Sequencing(Molecular and Cell Biology)

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概要

• 論文の詳細を見る

• In the present study, we examined nuclear DNA sequences in an attempt to reveal the relationships between Pueraria lobata (WILLD.) OHWI, P. thomsonii BENTH., and P. montana (LOUR.) MERR. We found that internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA are highly divergent in P. lobata and P. thomsonii, and four types of ITS with different length are found in the two species. On the other hand, DNA sequences of 5S rRNA gene spacer are highly conserved across multiple copies in P. lobata and P. thomsonii, they could be used to identify P. lobata, P. thomsonii, and P. montana of this complex, and may serve as a useful tool in medical authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii.

• 公益社団法人日本薬学会の論文
• 2007-01-01

著者

• Shaw Pang-chui

  Department Of Biochemistry And Institute Of Chinese Medicine The Chinese University Of Hong Kong

• Sun Ye

  Department Of Biochemistry And Institute Of Chinese Medicine The Chinese University Of Hong Kong:sou

• FUNG Kwok-Pui

  Department of Biochemistry and Institute of Chinese Medicine, The Chinese University of Hong Kong

• Fung Kwok-pui

  Department Of Biochemistry And Institute Of Chinese Medicine The Chinese University Of Hong Kong

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