Heart Preservation Using Continuous Ex Vivo Perfusion Improves Viability and Functional Recovery
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概要
- 論文の詳細を見る
Background Cold static storage (CS) is a proven preservation method for heart transplantion, yet early postoperative graft dysfunction remains prevalent, so continuous perfusion (CP) during ex vivo transport may improve viability and function of heart grafts. Methods and Results Canine hearts underwent CP (n=9) or CS (n=9) for 6h while intramyocardial pH was continuously monitored. Biopsies were assayed for ATP, caspase-3, malondialdehyde (MDA), and endothelin-1 (ET-1) levels at baseline, after preservation (t1), and after 1h of blood reperfusion on a Langendorff model (t2). Functional recovery was determined at ti by +dP/dt, -dP/dt, developed pressure, peak pressure and end-diastolic pressure. CP resulted in higher tissue pH and ATP stores and reduced caspase-3, MDA and ET-1 levels compared with CS at both t1 and t2. Post reperfusion recovery was significantly greater in CP vs CS for all myocardial functional parameters except end-diastolic pressure. Weight gain was significantly increased in CP vs CS at t1, but not at t2. Conclusions Low-grade tissue acidosis and energy depletion occur during CS and are associated with oxidative injury and apoptosis during reperfusion. CP attenuates these biochemical and pathologic manifestations of tissue injury, together with improved myocardial recovery, despite mild, transient edema.
- 社団法人日本循環器学会の論文
- 2006-12-20
著者
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Pierson Iii
Division Of Plant Pathology And Microbiology Department Of Plant Sciences College Of Agriculture And
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Miller Jr
Organ Recovery Systems Inc
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Griffith Bartley
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Pierson Iii
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Zhuo Jiachen
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Gullapalli Rao
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Ozeki Toshinaga
Division of Cardiac Surgery, University of Maryland School of Medicine and VA Medical Center at Balt
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Kwon Michael
Division of Cardiac Surgery, University of Maryland School of Medicine and VA Medical Center at Balt
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Gu Junyan
Division of Cardiac Surgery, University of Maryland School of Medicine and VA Medical Center at Balt
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Collins Michael
Division of Cardiac Surgery, University of Maryland School of Medicine and VA Medical Center at Balt
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Brassil John
Organ Recovery Systems, Inc
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Poston Robert
Division of Cardiac Surgery, University of Maryland School of Medicine and VA Medical Center at Balt
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Gu Junyan
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Kwon Michael
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Brassil John
Organ Recovery Systems Inc
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Poston Robert
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Collins Michael
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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Ozeki Toshinaga
Division Of Cardiac Surgery University Of Maryland School Of Medicine And Va Medical Center At Balti
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