走査型電子顕微鏡用試料作成のための第三ブチルアルコール凍結乾燥法

概要

論文の詳細を見る
INOUE and OSATAKE (1988) have recently developed a new tert.-butyl alcohol freeze-drying method for drying biological specimens of scanning electron microscope. Their method has come into wide use recently for drying zoological specimens. The specimens dried acccording to the new method seem to be in no way inferior to the critical-point dried specimens. But there have hardly any accomplishments with regard to the botanical specimens. So, we have intended to compare the botanical specimens dried by the new method with the critical-point dried specimens. And we could take the most exquisite photographs from the specimens produced according to the following procedures: (1) double fixation by 2.5% glutaraldehyde and 1% osmium tetroxide, each buffered at pH 7.2-7.4 with 0.1M phosphate buffer. (2) dehydration by tert. -butyl alcohol series (Tab. 1). (3) replacement with pure tert. -butyl alcohol. (4) freeze-drying with tert. -butyl alcohol (INOUE & OSATAKE 1988). The drying method is expected to become greatly available in future for drying the botanical specimens for scanning electron microscopy, although some further improvements are necessary to realized satisfactory and stable results from almost all botanical materials.
横浜国立大学の論文
1990-03-25