Fine Structure of Chromosomes in the Resting Nuclei of the Basidiomycete Lentinus edodes
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概要
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This study was carried out to reveal the fine structure of chromosomes at resting stage of secondary mycelia in a basidiomycete Lentinus edodes. The chromosomes were studied by cytochemical staining and electron microscopy. The results obtained may be summarized as follows: 1. The resting nuclei were classified into six types by their shapes oval, angular, or rod, and by the appearances that were partially condensed or wholly condensed. Most of the nuclei (83%) were found to have two to three condensed chromatic bodies. 2. By the thin section electron microscopy, the double-layered nuclear envelope with nuclear pores was validated, and electron dense chromosome regions and electron transparent chromosome regions were distinguished. The mean diameter of the chromosome fiber in the resting nucleus was about 65 Å. 3. The basic proteins (histones) were detected in chromosomes by alkaline fast green FCF staining and ammonical silver reaction. 4. By whole mount electron microscopy, the finest chromosome fibers were found to be 68 Å in mean diameter and be beadlike structure that was composed of spherical component (70 Å in mean diameter) and threadlike component (51 Å in mean diameter).
- 横浜国立大学の論文
- 1979-11-30
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関連論文
- Cytological Studies on Aster dimorphophyllus Franch. et Savat., with Special References to the Differentiation into Indivisus Form and Divisus Form
- Fine Structure of Chromosomes in the Resting Nuclei of the Basidiomycete Lentinus edodes