Simultaneous Determination of Triptolide, Wilforlide A and Triptonide in Human Plasma by High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry(Analytical Biochemistry)

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概要

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• A simple and sensitive method for the determination of triptolide, wilforlide A and triptonide in human plasma was developed and validated, using high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). Plasma samples were purified using solid-phase extraction (SPE) columns. The HPLC separation of the analytes was performed on a MACHEREY-NAGEL C_<18> column (2.0mm×125mm, 3μm), using 2.7mM formic acid containing 10mM ammonium acetate-acetonitrile (55:45, v/v) as mobile phase, with a flow-rate of 0.25ml/min. The compounds were ionized in the electrospray ionization (ESI) ion source of the mass spectrometer and were detected in the selected ion recording (SIR) mode. The calibration curves were linear in the 0.80-300ng/ml range for all the three analytes, and the limits of detection were 0.25, 0.40, and 0.35ng/ml for triptolide, wilforlide A, and triptonide, respectively. The average absolute recoveries for all the three analytes were above 81%. The methodology recoveries were greater than 91% and the relative standard deviations (RSD) of intra-day and inter-day were less than 15%. The developed method was successfully applied to the determination of triptolide, wilforlide A and triptonide concentration in patients' plasma after taking the medicament containing Tripterygium wilfordii HOOK. F.

• 公益社団法人日本薬学会の論文
• 2006-07-01

著者

• ZHANG Luyong

  National Drug Screening Center, China Pharmaceutical University

• Zhang Luyong

  National Center Of Drug Screening China Pharmaceutical University

• YAO Jincheng

  National Center of Drug Screening, China Pharmaceutical University

• JIANG Zhenzhou

  National Center of Drug Screening, China Pharmaceutical University

• HU Ling

  Clinical Pharmacy Research Institute, the Second Xiangya Hospital of Central South University

• Hu Ling

  Clinical Pharmacy Research Institute The Second Xiangya Hospital Of Central South University

• Yao Jincheng

  National Center Of Drug Screening China Pharmaceutical University

• Jiang Zhenzhou

  National Center Of Drug Screening China Pharmaceutical University:key Laboratory Of Drug Quality Con

• ZHAO Xuyuan

  Clinical Pharmacy Research Institute, the Second Xiangya Hospital of Central South University

• Zhao Xuyuan

  Clinical Pharmacy Research Institute The Second Xiangya Hospital Of Central South University

• Jiang Zhenzhou

  New Drug Screening Center China Pharmaceutical University

• Li Zhan

  New Drug Screening Center China Pharmaceutical University

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