In Vitro Antiviral Activity of Marine Sponges Collected Off Brazilian Coast(Pharmacognosy)

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概要

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• This paper describes the in vitro antiviral evaluation of 27 different marine sponges (Porifera) collected off Brazilian coastline in the search for novel drug leads. With these sponges aqueous and organic extracts were prepared and tested for anti-herpetic (HSV-1, KOS strain), anti-adenovirus (human AdV serotype 5) and anti-rotavirus (simian RV SA11) activities. The evaluation of the cytotoxicity and potential antiviral activity of these extracts were performed by using MTT assay. Results were expressed as 50% cytotoxicity (CC_<50>) and 50% effective (EC_<50>) concentrations, respectively, in order to calculate the selectivity indices (SI=CC_<50>/EC_<50>) of each extract. From the 40 sponge extracts tested, 17 extracts showed antiviral action in different degrees. The results concerning the antiviral activity were obtained by using three different strategies: (1) simultaneous assay, when sponge extracts were added to the cells at the same time of the viruses; (2) pre treatment assay, when sponge extracts were added to the cells 15h prior to the viruses infection; and (3) post treatment assay, when the viruses were added to the cells and remained during 2h prior to the addition of sponge extracts. The antiviral assays with HSV-1/KOS and AdV-5 showed more promising results when the pre treatment test was employed. In relation to the RV-SA11 virus, only the simultaneous assay showed antiviral activity. The extracts presenting the most promising results were the aqueous extracts of Cliona sp., Agelas sp.2, Tethya sp., Axinella aff corrugata, Polymastia janeirensis and Protosuberites sp., and these extracts deserve special attention in further studies.

• 2006-01-01

著者

• Lerner Clea

  Fundacao Zoobotanica Museu De Ciencias Naturais

• Silva Alexandra

  Labomtorio De Virologia Aplicada Da Ufsc Departamento De Ciencias Farmaceuticas Ccs Universidade Fed

• Simoes Claudia

  Laboratory Of Applied Virology Department Of Pharmaceutical Sciences Universidade Federal De Santa C

• Kratz Jadel

  Laboratory Of Applied Virology Department Of Pharmaceutical Sciences Universidade Federal De Santa C

• KRATZ Jadel

  Labomtorio de Virologia Aplicada da UFSC, Departamento de Ciencias Farmaceuticas, CCS, Universidade

• FARIAS Fabiane

  Faculdade de Farmdcia, Universidade Federal do Rio Grande do Sul (UFRGS)

• HENRIQUES Amelia

  Faculdade de Farmdcia, Universidade Federal do Rio Grande do Sul (UFRGS)

• SANTOS Josivete

  Departamento de Oceanografia, Universidade Federal de Pernambuco (UFPE)

• LEONEL Rosa

  Departamento de Sistemdtica e Ecologia, Universidade Federal da Paraiba (UFPB)

• MOTHES Beatriz

  Fundacao Zoobotanica, Museu de Ciencias Naturais

• BARARDI Celia

  Laboratorio de Virologia Aplicada da UFSC, Departamento de Microbiologia e Parasitologia, CCB, Unive

• SIMOES Claudia

  Labomtorio de Virologia Aplicada da UFSC, Departamento de Ciencias Farmaceuticas, CCS, Universidade

• Leonel Rosa

  Departamento De Sistemdtica E Ecologia Universidade Federal Da Paraiba (ufpb)

• Barardi Celia

  Department Of Microbiology And Parasitology Universidade Federal De Santa Catarina Ufsc

• Farias Fabiane

  Faculdade De Farmdcia Universidade Federal Do Rio Grande Do Sul (ufrgs)

• Mothes Beatriz

  Fundacao Zoobotanica Museu De Ciencias Naturais

• Santos Josivete

  Departamento De Oceanografia Universidade Federal De Pernambuco (ufpe)

• Henriques Amelia

  Faculdade De Farmdcia Universidade Federal Do Rio Grande Do Sul (ufrgs)

• da Silva

  Labomtorio de Virologia Aplicada da UFSC, Departamento de Ciencias Farmaceuticas, CCS, Universidade Federal de Santa Catarina (UFSC)

関連論文

• In Vitro Antiviral Activity of Marine Sponges Collected Off Brazilian Coast(Pharmacognosy)
• Evaluation of Anti-HSV-2 Activity of Gallic Acid and Pentyl Gallate(Medicinal Chemistry)

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