A Development of a Computer Program for Normalization of the Test Results by Fluorescence Enzyme-linked Immunosorbent Assay for Specific IgE Antibody

概要

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Fluorescence enzyme-linked immunosorbent assay (fluorescence-ELISA) is used to measure antigen specific IgE antibodies of allergic and parasitic diseases. However, the data obtained from it are often affected by varying conditions. We tried to normalize relative fluorescence unit (RFU) value from fluorescence-ELISA, which was measured under various conditions, using a computer program. From the curve of the RFU value obtained from the reference serum at 2 times serial dilution, the modified exponential function, y=a+be^<-cx>, was applied to a regression curve to which data points were fitted. We prepared a microcomputer program to satisfy this algebraic equation, and carried out some simulation tests in order to demonstrate the viability of this program. It was confirmed that there was no significant deviation from the data points from the regression curve. Also the modified exponential function was good enough for quantitative analysis of RFU value obtained from fluorescence ELISA under varying conditions. The function well normalized the decreases in results of individual data obtained from various dilution of -D-galactosidase conjugated anti-human IgE antibodies.
群馬大学の論文
1993-03-31