Effect of Shikonin on Human Breast Cancer Cells Proliferation and Apoptosis In Vitro
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概要
- 論文の詳細を見る
Shikonin, isolated from the plant Lithospermum erythrorhizon Sieb. Et Zucc, has been reported to induce apoptosis in several tumor cells. However, such effect of shikonin on human breast cancer cells has not been reported. Thus, in the present study, whether shikonin could induce MCF-7 human breast cancer cell apoptosis was investigated. The results showed that shikonin (2.5-80μM) induced MCF-7 cell death in a time- and dose-dependent manner, as measured by MTT assay. The IC_<50> of a 24h, 48h and 72h time course for MCF-7 cells was 7.4±0.4, 6.3±0.6 and 3.9±0.5μM, respectively. Cellular morphology observation showed that MCF-7 cells underwent marked apoptotic morphological changes upon treatment with 10μM shikonin compared with the untreated control. Flow cytometric analysis of shikonin-treated MCF-7 cells showed that the ratio of the apoptotic DNA fragmentation increased in a dose-dependent manner. The present study demonstrated for the first time that the cytotoxic effect of shikonin on MCF-7 cells underwent apoptosis process.
- 公益社団法人日本薬学会の論文
- 2006-12-01
著者
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Guo Tao
General Hospital Of Shenyang Military Region
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Hou Yue
Shenyang Pharmaceutical University:general Hospital Of Shenyang Military Region
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ZHAO Minghong
General Hospital of Shenyang Military Region
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WU Chunfu
Shenyang Pharmaceutical University
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HE Xiang
General Hospital of Shenyang Military Region
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HOU Yue
General Hospital of Shenyang Military Region
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