HIGH AMOUNT OF DINOPHYSISTOXIN-3 IN Mytilus chilensis COLLECTED IN SENO DE RELONCAVI, CHILE, DURING MASSIVE HUMAN INTOXICATION ASSOCIATED WITH OUTBREAK OF Vibrio parahaemolyticus

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概要

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• This study describes the detection of high amount of 7-O-acyl-derivative dinophysis-toxin-1 (Dinophysistoxin-3) in filter bivalves collected on February 2005 in the Seno de Reloncavi, Puerto Montt City, Southern Chile, in the same period of time where an intoxication episode was associated with the presence of Vibrio parahaemolyticus in shellfish. The Diarrhetic Shellfish Poisoning (DSP) mouse bioassay of mussel extract samples, performed as described for regulatory testing, were negative to DSP toxins. Therefore, the same mussel samples collected from 8 places of Seno de Reloncavi were then analyzed by the HPLC-FLD method with pre-column derivatization procedure for DSP toxins. The samples showed mainly 7-O-acyl-derivative dinophysistoxin-1 (Dinophysistoxin-3) in concentrations ranging from 190.3±6.8 to 311.1±4.8 ng of DSP toxin/g hepatopancreas and less amounts of Dinophysistoxin-1 ranging from 1.9±1.5 to 11.7±4.6 ng of DSP toxin/g hepatopancreas. After alkaline hydrolysis of the mussel extracts, 279.4±7.2 ng of Dinophysistoxin-1 /g hepatopancreas (mean±SEM, N=6) were found in mussel extracts (Zone 8). These data showed that these shellfish samples are contaminated with the ester form 7-O-acyl-derivatives of Dinophysisyoxin-1, far beyond the safe regulatory limit. This paper also shows a direct relation between lipid content in the mussel tissue extracts and the levels of Dinophysistoxin-3. The 7-O-acyl-derivative dinophysistoxin-1 ester was the only compound associated with DSP toxins detected in the shellfish samples, and in view of the fact that metabolic transformation of Dinophysistoxin-3 into Dinophysistoxin-1 in humans has recently been described in the literature, the consumption of shellfish contaminated with 7-O-acyl-derivatives dinophysistoxin-1 could be a major reason that explains the diarrhetic symptoms shown by the intoxicated patients.

• 日本トキシコロジー学会の論文
• 2006-10-23

著者

• Lagos Nestor

  Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive

• Garcia Carlos

  Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive

• SCHONSTEDT Valeria

  Laboratorio Bioquimica de Membrana, Departamento de Fisiologia y Biofisica, Facultad de Medicina, Un

• SANTELICES Juan

  Laboratorio Bioquimica de Membrana, Departamento de Fisiologia y Biofisica, Facultad de Medicina, Un

• Santelices Juan

  Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive

• Schonstedt Valeria

  Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive

関連論文

• First evidence of Okadaic acid acyl-derivative and Dinophysistoxin-3 in mussel samples collected in Chiloe Island, Southern Chile
• METABOLIC TRANSFORMATION OF DINOPHYSISTOXIN-3 INTO DINOPHYSISTOXIN-1 CAUSES HUMAN INTOXICATION BY CONSUMPTION OF O-ACYL-DERIVATIVES DINOPHYSISTOXINS CONTAMINATED SHELLFISH
• HIGH AMOUNT OF DINOPHYSISTOXIN-3 IN Mytilus chilensis COLLECTED IN SENO DE RELONCAVI, CHILE, DURING MASSIVE HUMAN INTOXICATION ASSOCIATED WITH OUTBREAK OF Vibrio parahaemolyticus

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