METABOLIC TRANSFORMATION OF DINOPHYSISTOXIN-3 INTO DINOPHYSISTOXIN-1 CAUSES HUMAN INTOXICATION BY CONSUMPTION OF O-ACYL-DERIVATIVES DINOPHYSISTOXINS CONTAMINATED SHELLFISH
スポンサーリンク
概要
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This paper describes for the first time a massive intoxication episode due to consumption of shellfish contaminated with 7-O-acyl-derivative dinophysistoxin-1, named Dinophysistoxin-3 (DTX-3). 7-O-acyl-derivative dinophysistoxin-1, a compound recently described in the literature, was found in shellfish samples collected in the Chilean Patagonia fjords. This compound does not inhibit Protein Phosphatases and also does not elicit the symptoms described for Diarrheic Shellfish Poisoning (DSP). The data showed here, give evidence of metabolic transformation of 7-O-acyl-derivative dinophysistoxin-1 (DTX-3) into Dinophysistoxin-1 (DTX-1, Methyl-Okadaic acid) in intoxicated patients. This metabolic transformation is responsible for the diarrheic symptoms and the intoxication syndrome showed by patients that consumed contaminated shellfish, which showed only the presence of 7-O-acyl-derivative dinophysistoxin-1. Patients fecal bacterial analysis for the presence of enteropathogens was negative and the mouse bioassay for DSP, performed as described for regulatory testing, was also negative. The HPLC-FLD and HPLC-MS analysis showed only the presence of DTX-3 as the only compound associated to DSP toxins in the contaminated shellfish samples. No other DSP toxins were found in the shellfish sample extracts. However, the patient fecal samples showed DTX-1 as the only DSP toxins detected in fecal. Moreover, the patient fecal samples did not show DTX-3. Since 7-O-acyl-derivative dinophysistoxin-1 (DTX-3) was the only compound associated to DSP toxins detected in the shellfish samples, an explanation for the diarrheic symptoms in the intoxicated patients would be the metabolic transformation of DTX-3 into DTX-1. This transformation should occur in the stomach of the poisoned patients after consuming 7-O-acyl-derivatives dinophysistoxin-1 (DTX-3) contaminated bivalves.
- 2005-12-21
著者
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Lagos Nestor
Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive
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Lagos Nestor
Laboratorio Bioquimica De Membrana Depto. Fisiologia Y Biofisica Fac. De Medicina Universidad De Chi
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Garcia Carlos
Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive
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Garcia Carlos
Laboratorio Bioquimica De Membrana Depto. Fisiologia Y Biofisica Fac. De Medicina Universidad De Chi
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Diaz Juan
Hospital Clinico Universidad De Chile Departamento De Cirugia
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Santelices Juan
Laboratorio Bioquimica De Membrana Departamento De Fisiologia Y Biofisica Facultad De Medicina Unive
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Santelices Juan
Laboratorio Bioquimica De Membrana Depto. Fisiologia Y Biofisica Fac. De Medicina Universidad De Chi
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TRUAN Dominique
Laboratorio Bioquimica de Membrana, Depto. Fisiologia y Biofisica, Fac. de Medicina, Universidad de
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LAGOS Marcelo
Laboratorio Bioquimica de Membrana, Depto. Fisiologia y Biofisica, Fac. de Medicina, Universidad de
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Lagos Marcelo
Laboratorio Bioquimica De Membrana Depto. Fisiologia Y Biofisica Fac. De Medicina Universidad De Chi
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Truan Dominique
Laboratorio Bioquimica De Membrana Depto. Fisiologia Y Biofisica Fac. De Medicina Universidad De Chi
関連論文
- First evidence of Okadaic acid acyl-derivative and Dinophysistoxin-3 in mussel samples collected in Chiloe Island, Southern Chile
- METABOLIC TRANSFORMATION OF DINOPHYSISTOXIN-3 INTO DINOPHYSISTOXIN-1 CAUSES HUMAN INTOXICATION BY CONSUMPTION OF O-ACYL-DERIVATIVES DINOPHYSISTOXINS CONTAMINATED SHELLFISH
- HIGH AMOUNT OF DINOPHYSISTOXIN-3 IN Mytilus chilensis COLLECTED IN SENO DE RELONCAVI, CHILE, DURING MASSIVE HUMAN INTOXICATION ASSOCIATED WITH OUTBREAK OF Vibrio parahaemolyticus