Modulation of Sperm Function during Sperm Transport in the Female

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概要

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• In the cow, sow and mare, a functional tubal sperm reservoir is established before ovulation to ensure availability of suitable numbers of viable spermatozoa for fertilization. Although identification of subpopulations reaching this reservoir has been attempted, it is still unclear whether this recruitment is programmed or fortituous. Those spermatozoa not reaching the oviduct are generally destroyed by phagocytosis of invading leukocytes. While the type of ejaculate differs in these species, seminal plasma proteins and/or the spermatozoa appear to act as leukocyte chemoattractant both in vitro and in vivo. Those spermatozoa in the sperm reservoir not only escape phagocytosis or rejection by the female immune system but sustain viability and potential fertilizing capacity by not capacitating or acrosome-reacting while residing in the oviduct. Sperm numbers in the reservoir diminish gradually in relation to ovulation, spermatozoa being continuously redistributed towards the upper isthmus. In vitro, only uncapacitated spermatozoa bind to epithelial explants, suggesting that the reservoir milieu modulates sperm capacitation. In vivo, most viable spermatozoa during preovulatory spontaneous standing oestrus are uncapacitated, with capacitation significantly increasing after ovulation. In either species, there seems to be different components of the oviductal fluid effecting capacitation, and bicarbonate appears to be common denominator for the membrane destabilizing changes that encompasses the first stages of the process. Such effects can be blocked or even reversed by co-incubation with isthmic fluid or specific glycosaminoglycans such as hyaluronan. Although the pattern of response to in vitro induction of sperm capacitation is similar for all spermatozoa, the capacity of response and its speed is very individual. Such diverse individual response to capacitation would not only confirm capacitation does not occur massively in the reservoir but clearly insures full sperm viability before ovulation and the presence of spermatozoa at different stages of capacitation in the upper oviduct, thus maximizing the chances of normal fertilization.CapacitationCowOviductSowMareSperm reservoirSperm transport

著者

• Rodriguez-martinez Heriberto

  Division Of Comparative Reproduction Obstetrics And Udder Health Department Of Clinical Sciences Fac

• Rodriguez-martinez Heriberto

  Division Of Comparative Reproduction Obstetrics And Udder Health Department Of Clinical Sciences Fac

関連論文

• Selection of Boar Spermatozoa Using Centrifugation on a Glycidoxypropyltrimethoxysilane-Coated Silica Colloid
• The Vanguard Sperm Cohort of the Boar Ejaculate is Overrepresented in the Tubal Sperm Reservoir In Vivo
• ウシ卵管上皮におけるヒアルロン酸受容体CD44の同定
• The Vanguard Sperm Cohort of the Boar Ejaculate is Overrepresented in the Tubal Sperm Reservoir In Vivo
• Modulation of Sperm Function during Sperm Transport in the Female
• Role of the Hyaluronan Receptor CD44 during Porcine Oocyte Maturation
• Role of the Hyaluronan Receptor CD44 During Porcine Oocyte Maturation
• Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium
• D-6. Modulation of sperm function during sperm transport in the female(Abstracts of the International Symposium on Recent Advances in Animal Science(IS-RAAS),Joint meeting of 2^ IS-AS and 3^ IS-IFS)

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