Seed coat sculpturing in Halophila

概要

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This study furnishes information on external morphology of the seed coats of selected Halophila. Fruiting plants of Halophila beccarii, H. ovalis, H. decipiens, Halophila sp. were collected from various locations around Malaysia and including Halophila stipulacea from Mauritus. Seeds extracted from mature fruits were fixed in 2.5% glutaradehyde. Fixed seeds were washed in 0.1M Sodium cacodylate buffer at 4℃ for 10 minutes. The washing procedure was repeated three times. The seeds were dehydrated in graded acetone series of 35%, 50%, 75%, 95% and 100% followed by in acetone and, dried in a Baltec 030 critical point dryer. After sputter-coating with gold, specimens were examined with a JEOL 6400 scanning electron microscope. Halophila seeds are sub-spherical and bluntly beaked at both ends. The Halophila seeds from different species showed diverse seed coats morphology with different sculpturing. Seeds of H. beccarii, Halophila ovalis, H. decipiens have reticulate coats with variations in microsculpturing within the reticulations. Seed coat of Halophila sp. is characteristically verrucose in appearance produced by warts or short peg-like projections. Seeds of Halophila stipulacea have reticulate seed coats and the boundaries of the reticulations are joined by peculiar sutures. These diverse seed coats morphology are illustrated by the electron micrographs taken from JEOL 6400 scanning electron microscope.
東京大学の論文
2006-04-28