Induced Formation of Enzymes in Cell-Free Preparations of Escherichia coli

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The fraction sedimenting at 29,000 ×g obtained from spheroplasts of E. coli with sonic vibration at low power or with digitonin treatment catalysed the increase in β-galactosidase activity. It was strongly suggested by experimental results that the increase in β-galactosidase activity by the fraction was based upon the net synthesis of protein and not due to the enzyme synthesis by intact spheroplasts contained in the fraction. The various behaviors of this fraction in the synthesis of β-galactosidase were demonstrated. This fraction was also capable of catalysing the increase in the activity of the other three enzymes. The particles sedimenting at 100,000 ×g was obtained from non-induced spheroplasts of an inducible strain disrupted with sonic vibration, which catalysed the increase in the activity of β-galactosidase in some degree. The increase in β-galactosidase activity by this particles required a soluble fraction, an inducer, a sourec of energy, nucleoside triphosphates, and was inhibited by RNase, DNase, or chloramphenicol, and accompanied with the increase in the amount of the protein precipitating with anti-β-galactosidase serum. These findings suggest strongly that the observed increase in β-galactosidase activity was based upon the net synthesis of protein. The preinduction of spheroplasts before the disruption of them enhanced the β-ga-lactosidase synthesis by the particles sedimenting at 100,000 ×g markedly, but enhanced that by the particles sedimenting at 29,000 ×g only slightly.
大阪府立大学の論文
1964-03-31

Induced Formation of Enzymes in Cell-Free Preparations of Escherichia coli

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