Visualization during Stick-Release and Plateau Responses of Collapsed DNA(Oscillation, Chaos and Network Dynamics in Nonlinear Science)
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概要
- 論文の詳細を見る
We have observed the elastic response of single DNA molecules and visualized it during the collapsing transition induced by trivalent cation, spermidine (SPD). A fluorescent dye, YOYO-1, was used for the visualization. While the contour length of DNA increases as the YOYO concentration increases, the contour length decreases as SPD concentration increases. This indicates that YOYO-SPD exchange occurs on the single DNA molecule. We have observed bright spots in the fluorescence intensity profile of collapsed DNA during stretching, which may correspond to the collapsed parts within the single DNA. The decrease of the intensity of the spots in stretching implies the mechanical unfolding of collapsed parts.
- 理論物理学刊行会の論文
- 2006-04-20
著者
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Sano Masaki
Univ. Tokyo Tokyo
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SANO Masaki
Department of Physics, Graduate School of Science, University of Tokyo
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Sano Masaki
Department Of Physics The University Of Tokyo
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Sano Masaki
Dept. Of Physics The Univ. Of Tokyo
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Murayama Yoshihiro
Dept. of Physics, The Univ. of Tokyo
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MURAYAMA Yoshihiro
Department of Physics, University of Tokyo
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ISHIDA Ryo
Department of Physics, University of Tokyo
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Ishida Ryo
Department Of Physics The University Of Tokyo
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Sano Masaki
Department Of Physics Graduate School Of Science The University Of Tokyo
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Murayama Yoshihiro
Department Of Physics The University Of Tokyo
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SANO Masaki
Department of Physics, University of Tokyo
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ISHIDA Ryo
Department of Physics, The University of Tokyo
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