ヒト歯肉におけるin vitroでのコラーゲン合成 : ^<14>C-プロリンを用いた光顕的オートラジオグラフィー

概要

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Five gingival biopsy specimens were obtained from maxillary or mandibular anteriors of each patient with periodontal disease. The weighed specimens were incubated with Krebs-Ringer phosphate buffer containing different kinds of amino acids and 3μCi of ^<14>C-labeled proline, for 0,30 minutes, 1, 2 and 4 hours at 37℃ in an atmosphere of 0_2. After the specimens incubated were immersed in 3ml of 60 mM cold proline solution, they were fixed in 10% formalin immediately. The paraffin sections were made (5μ) and stained with hematoxylin-eosin. The autoradiographs were taken and collagen synthesis was assessed by counting the silver grains per unit area in the apex of sulcular epithelium and the center of lamina propria. With an increase in the incubation time there was an increase in the counts of silver grain from sulcular epithelium, fibroblastic cells and collagen fibers. Possible explanation for the discrepancy in silver grain counts with time between fibroblastic cells and collagen fibers was discussed.
特定非営利活動法人日本歯周病学会の論文
1978-09-28