脱灰骨基質の骨形成能に関する基礎的研究

概要

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This experiment was designed to study the osteogenetic property in implants of allogeneic decalcified bone matrix gelatin. Long bones, excised from adult rats, were cut in length of 4 mm and treated sequentially. Extracted conditions were: 0.6 N HC1, 24 hr, 4℃; 8.0 M LiCl, 24 hr, 4℃; distilled water, 4 hr, 55℃. After lyophilization, decalcified bone matrix gelation were implanted in subcutaneous pouch of allogeneic recipients, and recovered for histologic and roentogenographic examination. The implanted matrix somehow induced migratory mesenchymal cells to differentiate into cartilage at 2 weeks, and to remodel and replace the deposits with lamellar bone and marrow between 3 to 4 weeks. The travecular interstices and old vascular channels were chiefly invaded with mesenchymal cells and histiocyte-like cells. Multinucleated giant cells were not detected. As calcification proceeded, area and intensity of von Kossa positive reaction were increased, whereas toluidine blue metachromasia and alcian blue positive region were reduced, and finally disappeared when ossification was completed.
特定非営利活動法人日本歯周病学会の論文
1978-06-28