Identification of QTL Controlling Flooding Tolerance in Reducing Soil Conditions in Maize (Zea mays L.) Seedlings(Genetic Resources Evaluation)
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the tolerance to flooding in reducing conditions of five maize inbred lines and identified a quantitative trait locus (QTL) for the trait. Flooding treatment with 0.1% to 0.4% starch solution for 14 d reduced soil redox potential to about -200mV, mimicking reducing conditions in soil. Treatment with 0.2% starch revealed wide varietal differences in dry matter production among the five maize inbred lines. We identified the QTL for flooding tolerance in reducing conditions in a population of 178 F_2 plants derived from a cross of inbred lines F1649 (tolerant) and H84 (sensitive). Flooding tolerance, evaluated as the degree of leaf injury following treatment with 0.2% starch solution, revealed wide variation in the F_2 population. Amplified fragment length polymorphism (AFLP) markers linked to flooding tolerance gene(s) were screened with 64 AFLP primer combinations using 15 of the 178 F_2 plants from each extreme representing the 'tolerant' and 'sensitive' plants, and found 11 AFLP markers associated with flooding tolerance. Of these, 10 co-segregated and were assigned to chromosome 1. Six SSR primer pairs around these markers were used to construct a linkage map. Composite interval mapping analysis revealed that a single QTL for degree of leaf injury was located on chromosome 1 (bin 1.03-4). Another QTL for flooding tolerance, evaluated as dry matter production under flooding with 0.2% starch, was located at the same position. These results suggest the potential to increase productivity by transferring flooding tolerance genes from F1649 to elite maize inbred lines.
- 日本作物学会の論文
著者
-
TAKAMIZO Tadashi
National Institute of Livestock and Grassland Science
-
Mano Yoshiro
National Institute of Agrobiological Sciences (NIAS)
-
Mano Yoshiro
Forage Crop Breeding Res. Team National Inst. Of Livestock And Grassland Sci.
-
Muraki Masanori
National Agricultural Research Center For Kyushu Okinawa Region
関連論文
- Screening of Regenerable Genotypes of Italian Ryegrass (Lolium multiflorum Lam.)(Genetic Resources Evaluation)
- Production of Transgenic Italian Ryegrass(Lolium multiflorum Lam. ) via Microprojectile Bombardment of Embryogenic Calli
- Estimation of genome size and physical mapping of ribosomal DNA in diploid and tetraploid guineagrass (Panicum maximum Jacq.)
- Hybridization between perennial ryegrass and Italian ryegrass in naturalized Japanese populations
- Optimization of culture conditions for plant regeneration of Panicum spp. through somatic embryogenesis
- The development of sequence tagged site marker sets for disease resistance gene analogs in maize (Zea mays L.)
- Construction of a High-density AFLP and SSR Map Using Recombinant Inbred Lines of Cultivated×Weedy Soybean
- Identification of QTL Controlling Flooding Tolerance in Reducing Soil Conditions in Maize (Zea mays L.) Seedlings(Genetic Resources Evaluation)
- QTL Mapping of Adventitious Root Formation under Flooding Conditions in Tropical Maize (Zea mays L.) Seedlings
- Agrobacterium tumefaciens-mediated transformation of forage-type perennial ryegrass (Lolium perenne L.)
- QTL mapping of above-ground adventitious roots during flooding in maize x teosinte "Zea nicaraguensis" backcross population
- Verification of QTL controlling root aerenchyma formation in a maize×teosinte "Zea nicaraguensis" advanced backcross population
- High-density linkage map around the root aerenchyma locus Qaer1.06 in the backcross populations of maize Mi29×teosinte "Zea nicaraguensis"
- Accurate evaluation and verification of varietal ranking for flooding tolerance at the seedling stage in barley (Hordeum vulgare L.)
- Breeding for flooding tolerant maize using "teosinte" as a germplasm resource
- Variation for the capacity to form root aerenchyma and selection for highly aerenchyma forming lines in the rare teosinte Zea nicaraguensis