Structural and Functional Properties of a 69-Kilodalton Outer Membrane Protein of Bordetella pertussis(I. Characterization of Virulence Factors of Bordetella pertussis)(Current Studies on Future Vaccines)

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概要

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• A-69-kDa outer membrane protein present on virulent Bordetella pertussis cells is recognized by the agglutinating monoclonal antibodies BPE3, BPD8, and BPE8. The amino acid composition of this protein, purified from heat extracts of B. pertussis BP353 cells, is different from that of the two major fimbrial antigens of B. pertussis, which is consistent with its being a nonfimbrial protein based on other criteria. Western blot analysis using the monoclonal antibody BPE3 demonstrated that a slightly larger but antigenically cross-reactive protein is also expressed by Bordetella bronchiseptica and Bordetella parapertussis. In addition, a large molecular weight species of about 180-kDa is found in outer membrane extracts of B. bronchiseptica which may represent a precursor form of the protein or indicate that the protein can exist as an oligomer. The monoclonal antibody BPD8 directed against the 69-kDa protein almost completely inhibited the enzymatic activity of adenylate cyclase purified from B. pertussis and also inhibited the intoxication of mammlian cells by this enzyme. Since little enzymatic activity was found associated with the purified 69-kDa protein, these data suggest a role for the 69-kDa protein in regulating the adenylate cyclase toxin of B. pertussis. An additional monoclonal antibody directed against the 69-kDa protein, BPE8, decreases lymphocytosis and delays death in mice receiving a respiratory challenge of virulent B. pertussis cells. These studies suggest that further investigation into the role of this protein as a protective antigen and vaccine candidate is warranted.

• 東海大学の論文
• 1988-09-22

著者

• Nguyen Nga

  Division Of Bacterial Products And Division Of Biochemistry And Biophysics Center For Biologics Eval

• Brennan Michael

  Laboratory Of Pertussis Center For Biologics Evaluation And Research Food And Drug Administration

• Li Zhong-ming

  Division Of Bacterial Products And Division Of Biochemistry And Biophysics Center For Biologics Eval

• BRENNAN Michael

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• SHAHIN Roberta

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• BURNS Drusilla

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• LIU Teh-Yung

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• GRAY Mary

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• HEWLETT Erik

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• MANCLARK Charles

  Division of Bacterial Products and Division of Biochemistry and Biophysics, Center for Biologics Eva

• BURNS Drusilla

  Laboratory of Pertussis, Center for Biologics Evaluation and Research, Food and Drug Administration

• Gray Mary

  Division Of Bacterial Products And Division Of Biochemistry And Biophysics Center For Biologics Eval

• Liu Teh-yung

  Division Of Bacterial Products And Division Of Biochemistry And Biophysics Center For Biologics Eval

• Hewlett Erik

  Division Of Bacterial Products And Division Of Biochemistry And Biophysics Center For Biologics Eval

• Shahin Roberta

  Division Of Bacterial Products And Division Of Biochemistry And Biophysics Center For Biologics Eval

• Burns Drusilla

  Laboratory Of Pertussis Center For Biologics Evaluation And Research Food And Drug Administration

• Manclark Charles

  Food And Drug Administration

関連論文

• Structural and Functional Properties of a 69-Kilodalton Outer Membrane Protein of Bordetella pertussis(I. Characterization of Virulence Factors of Bordetella pertussis)(Current Studies on Future Vaccines)
• Biochemical Properties of Pertussis Toxin(I. Characterization of Virulence Factors of Bordetella pertussis)(Current Studies on Future Vaccines)
• Outbreaks of Pertussis in the United States : The Wisconsin Experience(B. Vaccine Schedules: Their Pelationship to Current Epidemiology)(II. The Eradication of Pertussis: Current Unsolved Epidemiologic, Clinical and Public Health Problems which Impede Thi

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