細胞操作用マイクロマニピュレータの開発

概要

論文の詳細を見る
In this paper, a new micromanipulator has been developed for inserting a micropipet into an egg cell in a cell operation. Because the egg cell wall has strong elasticity, it is impossible to insert the micropipet into the egg cell by pressing the top of the micropipet on the egg cell wall. Therefore, it is necessary to make the micropipet vibrate continuously in the direction of insertion. The vibration principle for the micromanipulator is identical to that for an electrodynamic cone speaker utilized Fleming's left-hand rule in electromagnetic induction. This principle enables to change the magnitude of the vibration amplitude of a movable shaft of the micromanipulator by varying the frequency of an alternating electric current applied to a moving coil. And it is possible to obtain the vibration amplitude suitable to the elasticity of the egg cell wall and prevent the damage of the egg cell taken place in the cell operation. Consequently, present micromanipulator is expected to have excellent ability. In addition, simplified microscope image pickup system having easy operation has been composed of optical microscope, XYZ-table, CCD camera and TV monitor.
明治大学の論文