INTERLEUKIN-1 α INCREASES THE EXPRESSION OF INTEGRIN α_Vβ_3 IN HUMAN PANCREATIC CANCER CELLS
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概要
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Background and Aims: Pancreatic cancer is refractory to surgical treatment because of its high metastatic potential. We investigated the effect of IL-1 α on the expression of integrin α_Vβ_3 and tumor-associated angiogenesis in pancreatic cancer cell lines. Methods: We used three pancreatic cancer cell lines. The expression of integrin α_Vβ_3, mRNA and protein levels was examined by RT-PCR and flow cytometric analysis. The alteration of integrin α_Vβ_3 expression was measured using CELISA after treatment with rIL-1 α. A coculture system of HUVEC and fibroblasts was used to investigate tumor-associated angiogenesis. Results: Integrin cuand β_3 subunits were detected in all three cell lines both at mRNA and protein levels. The effect of the enhancement on integrin α_Vβ_3 expression induced by rIL-1 α was detected only in BxPC-3 cells, which possess a high potential to metastasize to the liver. Furthermore, a function blocking antibody to the α_Vβ_3 integrin inhibited tube formation associated with BxPC-3 cells. Conclusions: IL-1 α can increase the expression of the integrin α_Vβ_3 only in the pancreatic cancer cell line that exhibits a high potential to metastasize to the liver. Furthermore, the blockade of integrin α_Vβ_3 can inhibit tumor-associated angiogenesis, in vitro. These results suggested that IL-1 a is an important mediator of pancreatic cancer progression and that treatment with antibodies against integrin α_Vβ_3 may improve the prognosis of pancreatic cancer.
- 名古屋市立大学の論文
- 2005-05-31
著者
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竹山 廣光
Department Of Gastroenterological Surgery Nagoya City University Graduate School Of Medical Sciences
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松尾 洋一
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Science
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沢井 博純
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Science
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舟橋 整
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Science
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岡田 祐二
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Science
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真辺 忠夫
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Science
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栗本 昌明
Department Of Gastroenterological Surgery Nagoya City University Graduate School Of Medical Sciences
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舟橋 整
名古屋市立大学・臨床病態外科
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沢井 博純
名古屋市立大学消化器外科
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竹山 廣光
名古屋市立大学 大学院消化器外科
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山本 稔
Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Science
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