閉塞性黄疸肝の虚血再灌流時における高エネルギー燐酸化合物の変動に関する実験的検討 : 胆管十二指腸瘻モデルを用いた虚血前減黄効果について

概要

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We examined experimentally the effect of pre-ischemic biliary drainage on ischemia-reperfusion of obstructive jaundice by developing a new model in which pre-ischemic biliary drainage and subsequent hepatic ischemia-reperfusion examination can be done. Obstructive jaundice was achieved with clipping, and then unclipping, of the silastic tube for the choledochoduodenostomy. We analyzed levels of adenosine tri-phosphate (ATP) in liver tissue after normothermic hepatic ischemia and reperfusion for the following two groups. In obstructive jaundice (OJ) group, two weeks after clipping the silastic tube, an ischemia-reperfusion procedure was performed. In biliary drainage (BD) group, two weeks after clipping the silastic tube, obstructive jaundice was relieved with unclipping it. Bile was allowed to drain into the duodenum for a week and then an ischemia-reperfusion experiment was performed. With one week drainage in two week obstructive jaundice, serum total bilirubin decreased from 7.6 to 0.6 mg/dl. ATP levels were significantly higher in the BD group than in the OJ group only at 60 minutes after reperfusion (P<0.05). We developed successfully a new animal model in which the effects of preoperative biliary drainage were examined. These results suggest that pre-ischemic biliary drainage be useful because of higher ATP levels after reperfusion.
岐阜大学の論文
2003-03-31