ヒツジホワイトゴーストに対するアミノ酸の封入と調製方法の改良 : 可視紫外吸光光度法による半定量的な検討

概要

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In order to develop on utility of sheep waste bloods, author et al, studied inclusion of the 11 kinds of amino acids into the white ghosts from sheep blood by the absorption spectroscopy. There was a problem that the including degree of an amino acid was not evaluated in order that membrane proteins removed from the erythrocyte. Because the absorption band of the amino acid overlapped of the membrane protein's band at around 200nm. Therefore, we removed membrane proteins by the repeatable washing with phosphorus acid buffer solution, and found a better condition of supersonic processing. When including amino acids by the improved procedures on preparation and inclusion, the 11 kinds of amino acids could be confirmed to be included. Furthermore, the degrees of the inclusion were varied by the kinds of an amino acid, so probability of selective separations of the amino acid could be expected.
八戸工業高等専門学校の論文
2004-12-20