高速液体クロマトグラフィー及びウリカーゼ-UV法による血清尿酸測定法の比較及び問題点
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概要
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A high performance liquid chromatographic method presently under investigation by the Japan Society of Clinical Chemistry as a candidate reference method and an enzymatic method which was similarly investgated for determination of serum uric acid were comparatively studied employing an identical standard solution. Data by the two methods exhibited good correlation with no apparent difference in precision between the two method. However, we have encountered two control sera which pointed out problems for both methods. With the former method it will be necessary to check that the peak assigned to uric acid is solely attributable to uric acid by eliminating the uric acid peak employing uricase. As for the latter method, commercial control sera containing substances which show a strong absorption at the analytical wavelength would suffer in precision.
- 1993-03-15
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- 高速液体クロマトグラフィー及びウリカーゼ-UV法による血清尿酸測定法の比較及び問題点