Change of the Topography of Ventral Cell Surface during Spreading of Fibroblasts as Revealed by Evanescent Wave-Excited Fluorescence Microscopy : Effect of Contractility and Microtubule Integrity(<Special Issue>Bioengineering)

スポンサーリンク

概要

• 論文の詳細を見る

• Evanescent wave-excited fluorescence microscopy, which selectively probes the ventral membranes of cells adhered to glass substrate, was utilized to observe the change in the topography of the ventral plasma membranes of Swiss 3T3 fibroblasts during spreading. In the initial stage of the spreading (up to 2 hours after seeding), the ventral membrane was close (< 100 nm) to the substrate in the peripheral and the central regions. About 4 hrs after seeding, the ventral surface assumed a flat topography for a short period and then gradually became uneven, displaying streak pattern of cell-to-substrate contact (6-8 hours after seeding). By 24 hours after seeding, cells gained polygonal shape and most regions except for the focal adhesions were separated from the substrate. Within these well-spread cells actin stress fibers were found to emanate obliquely from the focal adhesions, as previously reported. When cells were grown in the presence of 2,3-butanedione monoxime (BDM), an inhibitor of actomyosin-based contraction of stress fibers and the cell, the ventral membranes in majority of the cells displayed flat topography, and the tilt of the stress fibers decreased. Cells grown in the presence of colchicine, a microtubule-depolymerizing agent also possessed flat ventral membrane and less tilted stress fibers. These results suggest, that the contraction of stress fibers and integrity of microtubules are important in the formation of the uneven topography of ventral membrane and the tilt of stress fibers.

• 社団法人日本機械学会の論文
• 2003-12-15

著者

• Hirata Hiroaki

  Cell Mechanosensing Project, SORST, Japan Science and Technology Agency

• Miyata Hidetake

  Physics Department, tohoku University

• Hirata Hiroaki

  Dept. Molecular Physiology Nips. Nins:sorst Cell-mechanosensing Project Jst

• Hirata Hiroaki

  Physics Department Graduate School Of Science Tohoku University

• Miyata Hidetake

  Physics Department Graduate School Of Science Tohoku University

• OHKI Kazuo

  Physics Department, Graduate School of Science, Tohoku University

• Ohki K

  Physics Department Graduate School Of Science Tohoku University

• Ohki Kazuo

  Physics Department Graduate School Of Science Tohoku University

関連論文

• 3P-182 C端LIM領域がzyxinの接着斑への機械的力に依存した局在の責任部位である(細胞生物学的課題(3),第46回日本生物物理学会年会)
• 3P-168 接着構造の試験管内再構成モデルの開発 : 力刺激による接着関連タンパク質の集積(細胞生物学的課題(3),第46回日本生物物理学会年会)
• 1P327 Mechanical force-dependent zyxin accumulation leads to actin polymerization at focal adhesions(11. Morphogenesis and cell adhesion,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 3P-149 細胞表面における後方輸送の平均二乗変位(MSD)を用いた解析(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
• Change of the Topography of Ventral Cell Surface during Spreading of Fibroblasts as Revealed by Evanescent Wave-Excited Fluorescence Microscopy : Effect of Contractility and Microtubule Integrity(Bioengineering)
• 3P-163 試験管再構成モデルを用いた力刺激による接着関連タンパク質の集積(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
• 1P-152 アクチン調節タンパク質zyxinのストレスファイバーへの張力依存的会合(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
• Tension-Dependent Formation of Stress Fibers in Fibroblasts : A Study Using Semi-Intact Cells
• Formation of Stress Fibers from Pre-Existing Actin Filaments driven by Mechanical Stress in Semi-Intact Cells(Micro- and Nano-biomechanics)

スポンサーリンク